Biosynthesis of silver and nickle oxide nanoparticles using polysaccharides fromchia seed mucilage and evaluating their biological applications | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Urooj Begum: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan

2. Afghana Firdous: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan

3. Aiman Khan: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan

4. Amjad Iqbal: Department of Food Science & Technology, Abdul Wali Khan University Mardan, Pakistan

5. Ayaz Ahmad: Department of Biotechnology, Abdul Wali Khan University, Mardan, Pakistan

Abstract:

Green synthesis of nanoparticles has gained attentions because of their diverse biological applications. The current paper report ecofriendly, and cost effective synthesis of silver (Ag) and nickle oxide (NiO) nanoparticles (NPs) using water-soluble polysaccharides extracted from chia (Salvia hispanica) seed mucilage. Chia seeds were soaked in autoclaved distilled water and the polysaccharides were extracted through 75% ethanol precipitation. The biosynthesized AgNPs and NiONps were confirmed through spectroscopic techniques. The UV visible spectrum of the AgNPs gives a sharp absorption peak at 473nm while that of NiONPs was recorded at 343nm. The antibacterial potential of the nanoparticles was evaluated using agar well diffusion method against five different human pathogenic strain. Results revealed that AgNPs were able to inhibit the growth of five human pathogenic Staphylococcus aureus (4.5mm ± 0.67mm), Escherichia coli (4.12mm ± 1.09mm), Klebsiella pneumonia (3.67mm ± 0.19mm), Salmonella typhi(5.16mm ± 1.23mm) and Shigella (2.9mm ± 0.85mm). The antioxidant potential was evaluated using DPPH radical scavenging assay. It was noted thatAgNPs and NiONPs exhibited prominet antioxidant activities by scavenging the DPPH free radical having with the EC50 value 88.53 and 90.23µg/mL, respectively. Furthermore, the invitro antidiabetic activity was evaluated using alpha amylase and alpha glucosidase inhibition assay. It was found that AgNPs and NiONPsshowed promising alpha amylase inhibitory potential having EC50 of 76.50µg/mL and 287µg/mL. These results suggest that water-soluble polysaccharides from chia seed mucilage has the potential to synthesized AgNPs and NiONPs with strong antimicrobial and antioxidant potential and could be exploited for further biomedical applications. This study concludes that utilizing mucilage polysaccharides could offers a sustainable source for green synthesis of bioactive nanomaterials.

Page(s): 117-117

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

antimicrobial , Nanoparticles , Chia , Polysaccharides , ntioxidant

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