Virus indexation of in vitro regenerated sugarcane plants. | [Pakistan Journal of Botany • 2009]

Author(s):

1. Shagufta Naz: Department of Botany, Lahore College for Women University, Lahore, Pakistan

2. Fayyaz Ahmad Siddiqui: AgriBiotech, 14-Km Multan Road, Lahore, Pakistan

3. Aamir Ali: Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan

4. Javed Iqbal: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

Abstract:

The present study reports the SCMV indexation of In vitro regenerated plants by infectivity assay, serology test and by transmission electron microscopy. The plants were developed by apical meristem, organogenesis and embryogenesis methods. For infectivity test sap of In vitro regenerated plants was inoculated on different cultivars of Sorghum. Out of 166 plants only 44 were SCMV+tive. These plants were further analyzed by using precipitin test. These plants also showed negative result for virus. A total of 17 randomly selected plants i. e, 2 from control, 5 each from apical meristem, organogenesis and somatic embryogenesis were processed for electron microscopy. The control plants showed virus particles. No virus particles were detected from plants derived from apical meristem. Two plants obtained from organogenesis and three from embryogenesis were identified with virus particles. All the SCMV indexed plants were grown in green house and monitored for mosaic symptoms at weekly intervals.

Page(s): 1931-1939

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 41, Issue: 4, Year: 2009

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