Author(s):
1. Rabia Iqbal Khan:
Institute of Industrial Biotechnology, Government College University,Lahore,Pakistan
2. Hamid Mukhtar:
Institute of Industrial Biotechnology, Government College University,Lahore,Pakistan
3. Umar Farooq Gohar:
Institute of Industrial Biotechnology, Government College University,Lahore,Pakistan
4. Syed Fahad Tahir:
Institute of Industrial Biotechnology, Government College University,Lahore,Pakistan
Abstract:
In this study, endophytic fungal strain HM-1 (Aspergillus sp.) has been reported to be capable of producing taxol. The wild strain was treated with physical (UV irradiation) and chemical (Nitrous acid and Ethidium bromide) mutagens to enhance taxol production. Mutant strains were subjected to surface culture fermentation at 30°C for 21 days. Taxol production was estimated quantitatively by HPLC while mycelial mass was calculated by the dry weight method. Results revealed that PM-5 mutant strain showed slightly increased production of taxol i.e. 99.44 ± 0.76 mg/L than 87.25 ± 0.5 mg/L of wild strain however, mycelial mass production was enhanced upto 10.25± 0.48 g/L of mutant strain C1 M-46 as compared to 7.75 ± 0.25 g/L of wild strain HM-1. 13 mutant strains gave low taxol production but high mycelial mass production as compared to wild strain HM-1. In addition to this, UV light, ethidium bromide, and nitrous acid had completely inhibited taxol production by 18 mutant strains. Results revealed that mutagens could enhance secondary metabolite production by fungi.
Page(s):
67-74
DOI:
DOI not available
Published:
Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 57, Issue: 2, Year: 2020
Keywords:
Taxol
,
Nitrous acid
,
high performance liacid chromatography HPLC
,
UV light
,
Ethidium bromide
,
Aspergillus sp
References:
References are not available for this document.
Citations
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