Abstract:
A significant horticultural herb-like plant with radical-shaped leaves and bluish-purple flowers is the Delphinium uncinatum Hook's & Thoms, belongs to the genus of Ranunculaceae. This plant's English name is Larkspur (Jadwar in Urdu), commonly known as Buttercup. Moreover, varieties of Delphinium plants have been cultivated and used as ornaments. Many studies exploited Delphiniums chemical composition and have tried to separate anthocyanin from Delphinium flowers. The research was also conducted on Delphinium alkaloids, particularly the diterpenoid alkaloid (DA). In this study, for the manufacture of biocompatible nanomaterials (Ag-Nps, Zn-Nps) via Delphinium uncinatum, simple benign biogenic techniques were used and then these biogenic and purchased chemically synthesized nanoparticles along with plant extract were tested in vitro and in-planta for their antibacterial effectiveness against the bacterial wilt pathogen Ralstonia solanacearum. Green synthesis of nanoparticles to control pathogens is one of the major concerns in modern bio-nanotechnology. Our findings showed that the biogenic and chemically silver and zinc oxide nanoparticles have 24.6, 28 nm and 100 nm, 23 nm average sizes respectively. The in vitro antibacterial study showed that the green synthesized and chemically silver, zinc nanoparticles, and whole plant extract inhibited the growth of Ralstonia solanacearum. Similarly, the in-planta study showed matching results with in vitro bioassay and the highest bacterial inhibition was observed. Disease severity was effectively reduced with plant extract < Ch-ZnNPs < Ch-AgNPs < GZnNPs <G-AgNP striated root area. Compared to Ch-ZnNPs, Ch-AgNPs, and plant extract treatments, G-AgNPs and GZnNps improved plant shoots, fresh biomass, and root length.
Page(s):
52-52
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 , Volume: 0, Issue: 0, Year: 2023