Type and Frequency of mutations in katG and rpoB genes in Multi-Drug Resistant Strains of Mycobacterium Tuberculosis Complex | [Pakistan Journal of Medical Research • 2017]

Author(s):

1. Muhammad Kashif Munir: PHRC TB Research Centre King Edward Medical University, Lahore, Pakistan

2. Muhammad Saqib: The University of Lahore, Pakistan

3. Sana Rehman: PHRC TB Research Centre King Edward Medical University, Lahore, Pakistan

4. Rizwan Iqbal: PHRC TB Research Centre King Edward Medical University, Lahore, Pakistan

5. Saqib Saeed: PHRC TB Research Centre King Edward Medical University, Lahore, Pakistan

6. Muhammad Aasim: NHRC Sheikh Zayed Hospital, Lahore, Pakistan

Abstract:

Background: Tuberculosis remains a major cause of morbidity and mortality in several parts of world. Genetic basis and mutations in katG and rpoB genes are responsible for isoniazid and rifampicin resistance in most of the cases of Mycobacterium tuberculosis. Objectives: To determine the mutations in katG and rpoB genes in confirmed multi-drug resistant tuberculosis isolates and to find the frequency of mutations Study design, settings and duration: This descriptive study was undertaken in PHRC TB research Centre, Department of Pulmonology, KEMU/Mayo Hospital Lahore. Polymerase chain reaction and genotyping was done at Institute of Molecular Biology and Biotechnology (IMBB), University of Lahore, Lahore from June 2013 to July 2014. Patients and Methods: A total of 100 acid-fast bacilli smear positive specimens of MDR TB suspects and rifampicin resistant on GeneXpert were collected. Drug susceptibility of isoniazid and rifampicin was carried out by standard drug proportion method. Gene amplification and sequencing was done to detect mutations in katG and rpoB genes. Results: A total of the 53% were females and 47% males with male to female ratio of 1:1.1. Mutations in rpoB Gene were found to be 98% of rifampicin resistant cases and in katG 76.7% of isoniazid resistant cases. Most of the mutations (60%) in rpoB Gene were observed on codon 531 while all the mutations in katG Gene were observed on codon 315. No novel mutation was found in this study. Conclusion: Mutation pattern of rpoB gene that confers rifampicin resistance is different to a little extent from other national and international studies while pattern is same for katG gene that confers isoniazid resistance. No novel mutation was observed in present study.

Page(s): 110-115

DOI: DOI not available

Published: Journal: Pakistan Journal of Medical Research, Volume: 56, Issue: 4, Year: 2017

Keywords:

Tuberculosis , mutations , MDR TB , katG and rpoB genes

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