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The Regulatory Networks Conferred by IFN-? in the Kidney of Takifugu rubripes.
Author(s)
Derong Kong College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Jun Cui College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Zhicheng Wang College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Lin Zang College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
He Sun College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Ziwen Hu College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Xin Li College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Xuemei Qiu College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Chen Jiang College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Haiying Liu College of Marine Science and Environment, Dalian Ocean University, Dalian, 116023, China
Tao Zhang Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China
Shengcong Liu Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China
Zhiqiang Jiang College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Xuesong Meng Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China
Xiuli Wang College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
Abstract
Takifugu rubripies is one of the most important economic fishes. Recently, because of various infectious diseases, production of T. rubripies has been seriously hindered. Interferon gamma (IFN-?) is critical for innate and adaptive immunity in fish. Here, a comparative RNA-Seq profiling study of the kidney of T. rubripes was performed to investigate the expression differences between the kidney samples with (Ts) and without (Cs) IFN-? infection. A total of 109,140,188 raw reads were generated from the Ts and Cs samples, including 106,717,920 clean reads. Additionally, a total of 19,124 expressed genes were identified from the two samples. Among these expressed genes, 292 genes showed significantly differential expression including 171 up-regulated and 121 down-regulated genes in the Ts sample. The GO, KEGG pathway enrichment and hierarchical clustering analyses indicated that the G-protein coupled receptor, Cytokine-cytokine receptor interaction, JakSTAT signaling pathways were likely to be affected by IFN-?. In addition, the protein-protein interaction (PPI) result showed that the MAPK6 was located at the center of this regulatory network involving IFN-?, suggesting that IFN-? may activate the MAPK signaling pathway. These results demonstrate the IFN-?-related molecular mechanisms in the kidney of T. rubripes, provide a basis for application of the recombinant IFN-? protein as a vaccine candidate against pathogen infection in aquaculture, and provide candidates to enhance pathogen-resistance in T. rubripes.
Publication Details
Page(s) 2189-2195
DOI 10.17957/IJAB/15.0758
Published Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 10, Year: 2018
Keywords
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