The DNA threat probing of some chromophores using UV/VIS spectroscopy | [World Journal of Biology and Biotechnology • 2023]

Author(s):

1. Shahab Khan: School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xian 710119, Shaanxi, China

2. Faizan Ur Rahman: Department of Chemistry, University of Malakand, 18800 Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan

3. Muhammad Zahoor: Department of Biohemistry, University of Malakand, 18800 Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan

4. Azmat Ul Haq: Department of Chemistry, University of Malakand, 18800 Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan

5. Abdul Bari Shah: Division of Applied Life Science, (BK21 Four) IALS, Gyeongsang National University, Jinju, RSK

6. Mudassir ur Rahman: Department of Chemistry, GDC Lundkhwar, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan.

7. Hamad Ur Rahman: Department of Chemistry, University of Malakand, 18800 Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan

Abstract:

Chromosphere such as triaryl methane family of dyes used in various industries can affect the body of living organism system upon the interaction with the most biochemical molecule is the DNA. In this research, the binding mode of triaryl methane family of chromophore dyes that is Bromothymol blue, Bromophenol blue, Bromocresol green and Malachite green with double strand DNA in phosphate buffer at pH 6.9 was investigated through UV/VIS spectroscopy. The study revealed that Bromothymol blue undergo noncovalent interaction with DNA, bromocresol green interact through groove binding and malachite green and bromophenol blue interact through intercalative and partially through electrostatic mode of interaction with DNA. The studies on the binding nature of chemicals/dyes, which are regarded as small molecules to DNA are important and fundamental issues in life sciences. These dyes were found to be more toxic and care should be taken during disposing them off. Proper disposal of these dyes is needed for safe environment and healthy life.

Page(s): 19-22

DOI: DOI not available

Published: Journal: World Journal of Biology and Biotechnology, Volume: 8, Issue: 2, Year: 2023

Keywords:

Malachite Green , Bromophenol Blue , Bromothymol blue , phosphate buffer , bromocresol green

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