Virulence Determination in Invasive and Commensal Staphylococcus Epidermidis | [Journal of Pioneering Medical Sciences • 2024]

Author(s):

1. Hind Hamid Muneam: Department of Biology, College of Science, University of Baghdad,Baghdad,Iraq

2. May Talib Flayyih: Department of Biology, College of Science, University of Baghdad,Baghdad,Iraq

Abstract:

Aims research primarily focuses on the virulence factors in Staphylococcus epidermidis isolates and their role in human disease. Methods: 15 Staphylococcus epidermidis Isolates were obtained from urine, wound, and blood samples from various hospitals in Baghdad. Additionally, 15 Staphylococcus epidermidis isolates were collected from the urine and ifngerprints of healthy individuals in Baghdad from July 2021 to January 2022. The resistance of the isolates to antibiotics was evaluated by conducting the disk diffusion test, which involves placing antibiotic-containing disks on a culture plate to observe the extent of bacterial growth inhibition around each disk. The antibiotics tested included Vancomycin (VA- 30µg), cefoxitin (CFX- 30µg), gentamicin (CN-10µg), tetracycline (TE-10 µg), levofloxacin (LEV- 5µg), oxacillin (OX- 5µg), penicillin G (P10 µg), Rifampin (RA- 5µg), and clindamycin (DA-10 µg). The frequency of virulence genes (fdh, mecA, clf, IgG, sesI) was determined using PCR assay. Results: A total of 30 S. epidermidis isolates were detected by traditional biochemical tests, and Viteck 2 had higher rates of resistance to cefoxitin (80%), oxacillin (90%), penicillin (86.6%), Clindamycin (60%), Gentamycin (76.6%) levofloxacin (80%) Tetracycline (26.6%). The most effective antibiotics are vancomycin (90%) and Rifampin (86%). Moreover, the detection 3/15(20%), 12/15(80%), of commensal marker fdh, and 3/15(20%),1/15(6.6%) of invasive marker sesI genes 11/15(73.3%), 14/15(93.3%) while other virulence genes mecA, clf, IgG detected and 6/15(40%), 7/15(46.6%), 9/15(60%), 14/15(93.3%) in clinical and healthy individual isolates, respectively.

Page(s): 68-74

DOI: 10.61091/jpms202413412

Published: Journal: Journal of Pioneering Medical Sciences, Volume: 13, Issue: 4, Year: 2024

Keywords:

Staphylococcus epidermidis , mecA , IgG , fdh , clf , sesI

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