ZOO-1689: Remedy of Fipronil-Induced Hepatoxicity with Ascorbic Acid and Silver Nanoparticles in Chicken | [4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025 • 2025]

Author(s):

1. Fareeha Ambreen: Department of Zoology, Rawalpindi Women University,,Pakistan

2. Laiba Saifullah: Department of Zoology, Rawalpindi Women University,,Pakistan

3. Jawaria Tufail: Department of Zoology, Rawalpindi Women University,,Pakistan

4. Samina Qamer: Department of Zoology, Rawalpindi Women University,,Pakistan

5. Rabea Ejaz: Department of Zoology, Rawalpindi Women University,,Pakistan

6. Anas Bilal: Department of Zoology, Rawalpindi Women University,,Pakistan

Abstract:

The current study aimed to investigate the toxic effects of Pyrazole and Fipronil on liver function in developing chicks, and the protective effects of Ascorbic acid and Silver nanoparticles. Chicks measuring 20-30g, aged 2-3 days, were collected from the hatchery and separated into nine groups. Doses were administered orally every day for four weeks. Group I was control group treated with 0.9% NaCl. Group II was administered Pyrazole (25 mg/kg b.w.). Group III was given 20 µl of silver nanoparticles (AgNP) each animal. Group IV was administered Pyrazole (25 mg/kg b.w.) and Ascorbic acid (250 mg/kg b.w). Group V received Ascorbic acid (250 mg/kg b.w). Group VI received Fipronil (4.5 mg/kg b.w.). Group VII received Fipronil (4.5 mg/kg body weight) and Ascorbic acid (250 mg/kg body weight). Group VIII was administered with AgNP (20 µl/animal) and fipronil (4.5 mg/kg body weight). Group IX received AgNP (20 µl/animal) and pyrazole (25 mg/kg b.w). Animals underwent dissection after the last dose administration and blood was collected for liver function assays including ALT, AST, and ALP. Results showed that Pyrazole treated group had slightly higher body weight (p<0.0001) in the 4 weeks-time of treatment, whereas Fipronil group exhibited considerably lower weight gain (p<0.05). ALT, AST, and ALP levels were significantly increased (p<0.0001) in the Pyrazole and Fipronil exposed groups. Treatment with Ascorbic acid (p<0.0001) and silver nanoparticles (p<0.05) resulted in better weight gain and enzyme levels were comparable to the control. There was a substantial link between enzyme elevation and weight suppression. The study found that Pyrazole and Fipronil produced hepatotoxicity in chicks, but Ascorbic acid and Silver nanoparticles reduced liver toxicity.

Page(s): 202-202

DOI: DOI not available

Published: Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025

Keywords:

hepatotoxicity , Silver nanoparticles , ALT , ALP , AST , Fipronil

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