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The nicotinamide nucleotide transhydrogenase gene could impair blood glucose level stability and increase basal metabolism.
Author(s):
1. Fathia Soudy: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Department of Genetics and Genetic Engineering, Benha University, Moshtohor, Toukh, Kalyobiya 13736, Egypt;
2. Akhtar Rasool Asif: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; College of Veterinary and Animal Science, Jhang, Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan;
3. Yuanxin Miao: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University,Wuhan, Hubei Province 430070,China
4. Lu Jing: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University,Wuhan, Hubei Province 430070,China
5. Yu Luan: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China;
6. Ali Haider Saleem: College of Veterinary and Animal Science, Jhang, Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan;
7. Ali Zohaib: Wuhan Institute of Virology of Chinese Academy of Sciences, Wuhan, Hubei Province 430071, China;
8. Sayyed Aun Muhammad: College of Veterinary and Animal Science, Jhang, Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan;
9. Zulfiqar Ahmad: Department of Food Science and Technology, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur- Pakistan.
10. Shuhong Zhao: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China;
11. Xinyun Li: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University,Wuhan, Hubei Province 430070,China
Abstract:
C57BL/6J (B6J) is the most widely used mouse strain for metabolic research. It carries a spontaneous mutation in the nicotinamide nucleotide transhydrogenase (Nnt) gene. This study compared blood glucose levels in B6J and Kunming (Km) mice as control. It was observed that blood glucose levels in B6J and Km mice at 4 weeks of age decreased post feeding and reached the lowest level at 24 hr of fasting. Blood glucose was significantly higher in B6J mice than that in Km mice at 0 hr, 2 hr and 10 hr of fasting. In addition, the correlation between the Nnt gene, growth traits and the feed conversion efficiency ratio (FCR) in (B6J × Km) F2 generation (N = 342) was also analyzed. We found that Nnt was significantly associated with body weight at 3 weeks (IBW) (P<0.01) and 5 weeks (FBW) (P<0.05) and average metabolic body weight (AMBW) (P<0.01) but was not associated with average daily feed intake (AFI), average daily gain (ADG) and FCR. Furthermore, qRT- PCR revealed that the expression levels of Glut-1, Glut-2, Akt-1, Irs-1 and Ucp-2 genes were significantly different in high and low-FCR mice. Our study offers novel evidence of the roles of Nnt gene in metabolism and growth.
Page(s): 603-609
Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 55, Issue: 3, Year: 2018
Keywords:
mice , blood glucose , familial glucocorticoid deficiency , autosomal recessive disorder , feed conversion ratio , glucose metabolism
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