Wheat genotypes respond differently under polyethylene glycol (peg) induced drought stress during germination and early seedling growth stages | [Pakistan Journal of Botany • 2025]

Author(s):

1. MST MASUMA AKHTER: Bangladesh Wheat and Maize Research Institute, Dinajpur-5200, Bnagladesh; Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University Dinajpur-5200, Bnagladesh;

2. MA HASAN: Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University Dinajpur-5200,Bnagladesh,

3. MM BAHADUR: Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University Dinajpur-5200,Bnagladesh,

4. MR ISLAM: Regional Agricultural Research Station (Bangladesh Agricultural Research Institute),Ishurdi, Pabna,Bangladesh

5. MUHAMMAD AAMIR IQBAL: Department of Chemical Engineering, Louisiana Technical University,Ruston LA 71272,USA

6. WALID SOUFAN: Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia

7. KHANDAKAR AURIB: Department of Disaster Science and Climate Resilience, University of Dhaka,Dhaka,Bangladesh

8. TANJILA AKHTER: Institute of Water and Flood Management, Bangladesh University of Engineering and Technology, Dhaka, Bangladesh

9. SIPAN SOYSAL: Department of Field Crops, Faculty of Agriculture, Siirt, Türkiye

10. AYMAN ELSABAGH: Department of Field Crops, Faculty of Agriculture, Siirt, Türkiye;Department of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt

11. MOHAMMED HENIESH: Department of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt

12. MOHAMMAD SOHIDUL ISLAM: Department of Agronomy, Hajee Mohammad Danesh Science and Technology University Dinajpur-5200, Bnagladesh

Abstract:

Water scarcity has emerged as one of a critical environmental constriction that negatively affect wheat growth, development and yield in Bangladesh. Identification of drought tolerant genotypes is crucial at drought sensitive initial stages of plant growth especially germination and seedling growth stages. To cater this issue, 30 wheat genotypes were subjected to polyehelene glycol (PEG 6000) induced drought stress levels of 0, -2.0 and -4.0 Mpa (osmotic stresses) and their response was tested at the germination and seedling growth stages. The experimental set up was Completely Ramdomized Design (CRD) with five replications. The results revealed that the rate of germination (RG) among the genotypes varied significantly with the different water potential levels, and the RG decreased with the advancement of stress. The stress tolerance index (STI) values based on RG were > 0.900 in wheat cultivars of Shatabdi, BARI Gom 25, BAW 1118, BAW 1151, BAW 1161, E 2, E 18, E 30, E 34 and E 38under severe water deficit stress, while the values were 0.980 STI values whereas, BARI Gom 26, BARI Gom 27, E 24, E 28, E 42, BAW 1130, BAW 1140 and BAW 1168 produced 0.920) regarding the germination vigour index (GVI) under higher water stress were observed in BARI Gom 25, E 18, E 23, E 34, E 38, BAW 1118, BAW 1161 and BAW 1170, and very lower STI values ( 0.550) indicating tolerant genotypes and the genotypes Sourav, BARI Gom 26, BARI Gom 28, BAW 1130, BAW 1151, BAW 1168, E 2, E 3, E 28 and E 29demonstrated lower STI values (< 0.450) designating susceptible genotypes. As water deficit stress increased, the shoot weight (g) of wheat genotypes decreased. Notably, genotypes Shatabdi, BARI Gom 25, E 18, E 34, E 38, and BAW 1118 exhibited Stress Tolerance Index (STI) values greater than 0.700. In contrast, genotypes BARI Gom 26, E 2, E 23, E 29, BAW 1130, BAW 1140, BAW 1151, BAW 1157, and BAW 1161 displayed STI values lower than 0.550. The genotypes showing higher and lower STI values denoted water stress tolerant and susceptible genotypes, respectively.

Page(s): 21-36

DOI: 10.30848/PJB2025-1(23)

Published: Journal: Pakistan Journal of Botany, Volume: 57, Issue: 1, Year: 2025

Keywords:

wheat , germination , Polyethylene Glycol , Drought stress

References:

[1] Abbas S.F.,Bukhari M.A.,Raza M.A.S.,Abbasi G.H.,Ahmad Z.,Alqahtani M.D.,Almutairi K.F.,Abd E.F.M.,Iqbal M.A. .2023 .Enhancing drought tolerance in wheat cultivars through nano-ZnO priming by improving leaf pigments and antioxidant activity. Sustainability, 15 : 5835.

[2] Ahmad M.,Shabbir G.,Minhas N.M.,Shah M.K.N. .2013 .Identification of drought tolerant wheat genotypes based on seedling traits. Sarhad J. Agric., 29(1) : 21-27.

[3] Ahmad Z.,Waraich E.A.,Tariq R.M.S.,Iqbal M.A.,Ali S.,Soufan W.,M.M. Hassan M.S.,Islam A. El,Sabagh A. El .2021 .Foliar applied salicylic acid ameliorates water and salt stress by improving gas exchange and photosynthetic pigments in wheat. Pak. J. Bot., 53(5) : 1553-1560.

[4] Ahmed A.M.,Wais A.H.,Ditta A.,Islam M.R.,Chowdhury M.K.,Pramanik M.H.,Ismaan H.N.,Soufan W.,Sabagh A. El,Islam M.S. .2024 .Seed germination and early seedling growth of sorghum (Sorghum bicolor L. Moench) genotypes under salinity stress. Polish J. Environ. Stud., 10(5) : 1-14.

[5] Akhter M.M.,Sabagh A. EL,Alam M.N.,Hasan M.K.,Hafez E.,Barutçular C.,Islam M.S. .2017 .Determination of seed rate of wheat (Triticum aestivum L.) varieties with varying seed size. Sci. J, 6(3) : 161-167.

[6] Akhter M.M.,Hossain A.,Teixeira da Silva J.A.,Timsina J.C.,Islam M.S. .2019 .Growth and yield of five irrigated spring wheat varieties as influenced by seeding rate in Old Himalayan Piedmont Plain of Bangladesh. Songklanakarin J. Sci. Technol, 41(2) : 389-396.

[7] Almaghrabi O.A. .2012 .Impact of drought stress on germination and seedling growth parameters of some wheat cultivars. Life Sci. J, 9(1) : 590-598.

[8] Ashraf M.H.,Naqvi A.H.,Khan A.H. .1996 .Evaluation of four screening techniques for drought tolerance in wheat (Triticum aestivum L.). Acta Agron. Hungarica, 44(3) : 213-220.

[9] Awan S.A.,I. Khan S.A.,Rizwan M.,Zhang X.,Brestic M.,Khan A. .2021 .Exogenous abscisic acid and jasmonic acid restrain polyethylene glycol-induced drought by improving the growth and antioxidative enzyme activities in pearl millet. Physiol Plant, 172 : 809-819.

[10] Azam M.G.,Islam M.S.,Hasan K.,Choudhury M.K.,Alam M.J.,Shaddam M.O.,El-Sabagh A. .2018 .Effect of storage containers and length of storage on the germination, moisture content and pest infestation of wheat seed. Cercetari Agron, 51(3) : 13-23.

[11] Barutcular C.,M. Yildirim M.,Koc H.,Dizlek C.,Akinci A. EL,Sabagh H.,Saneoka A.,Ueda M.S.,Islam I.,Toptas O.,Albayrak A.,Tanrikulu A. . .Quality traits performance of bread wheat genotypes under drought and heat stress conditions. Fres. Environ. Bull., 25 : 6159-6165.

[12] Botwright T.L.,Condon A.G.,Rebetzke G.J.,Richards R.A. .2002 .Field evaluation of early vigour for genetic improvement of grain yield in wheat. Aust. J. Agric. Res., 53 : 1137-1145.

[13] Chowdhury M.K.,Hasan M.A.,M.M. Bahadur M.R.,Islam M.A.,Hakim M.A.,Islam M.S. .2021 .Evaluation of drought tolerance of some wheat (Triticum aestivum L.) genotypes through phenology, growth, and physiological indices. Agronomy, 11 : 1792.

[14] Copeland L.O.,Minneapolis L.O. .1976 .Principles of seed science and technology. Burgess Pub, : 164-165.

[15] Dadbakhsh A. ,Yazdansepas A.,Ahmadizadeh M. .2011 .Study drought stress on yield of wheat (Triticum aestivum L.) genotypes by drought tolerance indices. Adv. Envid. Biol, 5(7) : 1804-1810.

[16] Dhanda S.S.,Sethi G.S.,Beh R.K. .2004 .Indices of drought tolerance in wheat genotypes at early stages of plant growth. J. Agron. Crop Sci., 190(1) : 6-12.

[17] Digby P.,Galway N.,Lane P. .1989 .Genstat 5: A second course. Oxford Sci. Publication, : 103-108.

[18] Dodig D.,Stanisavljevic R. .2015 .Wheat seedlings growth response to water deficiency and how it correlates with adult plant tolerance to drought. J. Agri. Sci., 153(3) : 466-480.

[19] Sabagh El,Barutçular C.,Islam M.S. .2017 .Relationships between stomatal conductance and yield under deficit irrigation in maize (Zea mays L.). J. Exp. Biol. Agri. Sci., 5(1) : 14-20.

[20] Sabagh EL,A. EL,Hossain A.,Barutçular C.,Islam M.S.,Awan S.I.,Galal A.,Iqbal A.,Sytar O.,Yildirim M.,Meena R.S.,Fahad S.,Najeeb U.,Konuskan O.,Habib R.A.,Llanes A.,Hussain S.,Farooq M.,Hasanuzzaman M.,Abdelaal K.H.,Hafez Y.,Cig F.,Saneoka H. .2019 .Wheat (Triticum aestivum L.) production under drought and heat stress-adverse effects, mechanisms and mitigation: a review. Appl. Ecol. Environ. Res., 17(4) : 83078332-8332.

[21] Sabagh EL,A. EL,Islam M.S.,Skalicky M.,Raza M.A.,Singh K.,Hossain M.A.,Hossain A.,Mahboob W.,Iqbal M.A.,Ratnasekera D.,Singhal R.,Ahmed S.,Kumari A.,Wasaya A.,Sytar O.,Brestic M.,Cig F.,Erman M.,M. M.,Arshad A.U. .2021 .Salinity stress in wheat (Triticum aestivum L.) in the changing climate: Adaptation and Management Strategies. , 10 : 661932.

[22] Fard A.K.,Sedaghat S. .2013 .Evaluation of drought tolerance indices in bread wheat recombinant inbred lines. Europ. J. Exp. Biol, 3(2) : 201-204.

[23] Farooq M.,Hussain M.,Usman M.,Farooq S.,Alghamdi and K.H.M. Siddique S.S. .2018 .Impact of abiotic stresses on grain composition and quality in food legumes. J. Agric. Food Chem, 66 : 8887-8897.

[24] González L.,Ayerbe L. .2011 .Response of coleoptiles to water deficit: growth, turgor maintenance and osmotic adjustment in barley plants (Hordeum vulgare L.). Agri. Sci., 2 : 159-166.

[25] Goudarzi M.,Pakniyat H. .2008 .Evaluation of wheat cultivars under salinity stress based on some agronomic and physiological traits. J. Agric. Soci., 4 : 35-38.

[26] Grzesiak S.K.A.,Janowiak F.,Rzepka A.,Hura T. .2012 .The relationship between seedling growth and grain yield under drought conditions in maize and triticale genotypes. Acta Physiol. Plant, 34 : 1757-1764.

[27] Hafeez A,Ali S.,Javed M.A.,Iqbal R.,Khan M.N.,Çiğ F.,Ali B. .2024 .Breeding for water-use efficiency in wheat: Progress, challenges and prospects. Mole. Biol. Rep, 51(1) : 429.

[28] Hair J.R.,Anderson R.E.,Tatham R.L.,Black W.C. .1995 .Multivariate data analysis with readings (4th ed. , : 498.

[29] Hossain M.M.,Alam M.N.,Hakim M.A.,Islam M.Z.,Al M.A.,Mamun M.A.,Islam R.,Amin M.F.,Mustarin K.,Ekram M.S.B.,Sharmin S.,Islam M.S.,Akter S.,Abedin M.T.,Masud M.Z. .2023 .Development of short duration, tolerance to high temperature and bipolaris leaf blight, and moderately susceptible to blast disease of wheat genotype with trials in various Agroecological Zones in Bangladesh. Int. J. Sci. Res, 10(9) : 395-410.

[30] Islam M.R.,Sarker B.C.,Ditta A.,Alam M.A.,M.M. Akhter W.,Soufan K.,Rajendran A. EL,Sabagh D.,Ratnasekera C.,Chukwuma C.C.,Ogbaga M.O.,Shaddam M.S.,Islam M.S. .2024 .Discrimination of mung bean (Vigna radiata L.) genotypes exposed to PEG-induced water deficit reveals the selection criteria for improved breeding from germination to seedling development. Pol. J. Environ. Stud., 10(5) : 1-12.

[31] Islam M.S.,Islam M.R.,Hasan M.K.,Golam Hafeez A.S.M.,Hosen M.M.,Saneoka H.,Ueda A.,Naz M.,Barutçular C.,Lone J.,Raza M.A.,Chowdhury M.K.,Sabagh A. El,Erman M. .2022 .Soybean and sustainable agriculture for food security. SoybeanRecent Advances in Research and Applications. IntechOpen, : 1-26.

[32] Jahanbin M.A.,Roshdi M.,Zaefizadeh M. .2012 .Effects of osmotic stress on germination and germination indices of synthetic wheat. Ann. Biol. Res., 3(2) : 995-999.

[33] Jajarmi V.,Eng V. .2009 .Effect of water stress on germination indices in seven wheat cultivar. World Aca. Sci, 49 : 105-106.

[34] Kamran M.,Wang D.,Xie K.,Lu Y.,Shi C.,Sabagh A.E.,Xu P. .2021 .Pre-sowing seed treatment with kinetin and calcium mitigates salt induced inhibition of seed germination and seedling growth of choysum (Brassica rapa var. Ecotoxicol. Environ. Safe, 227 : 112921.

[35] Kaydan D.,Yagmur G. .2008 .Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. , 7(16) : 2862-2868.

[36] Khayantnezhad M.,Gholamin R.,Jamaati- S.,Zabihi- R. .2010 .Study of water stress effect on wheat genotypes on germination indexes. Mid-East J. Sci. Res, 6(6) : 657-660.

[37] Krishnasamy D.V.,Seshu D.V. .1990 .Germination after accelerated aging and associated characters in rice varieties. Seed Sci. Tech, 18 : 353-359.

[38] Mahalanobis P.C. .1936 .On the generalized distance in statistics. Proc. Nat. Sci. Ind, : 49-55.

[39] Michel B.E.,Physiol Plant .1983 .Evaluation of the Water Potentials of Solutions of Polyethylene Glycol 8000. , 72 : 66-70.

[40] Moud A.M.,Maghsoudi K. .2008 .Application of coleoptile growth response method to differentiate osmoregulation capability of wheat (Triticum aestivum L.) cultivars. Res. J. Agron., 2 : 36-43.

[41] Noorka I.R.,Khaliq I.,Kashif M. .2007 .Index of transmissibility and genetic variation in spring wheat seedlings under water deficit conditions. Pak. J. Agril. Sci., 44(4) : 604-607.

[42] Qayyum A,Ahmad S.,Liaqat S.,Malik W.,Saeed H. M.,Hanif M. .2012 .Screening for drought tolerancein maize (Zea mays L.) hybrids at an early seedling stage. Afr. J. Agric. Res., 7(24) : 3594-3604.

[43] Raza M.A.S.,Saleem M.F.,Khan I.H.,Jamil M.,Ijaz M.,Khan M.A. .2012 .Evaluating the drought stress tolerance efficiency of wheat (Triticum aestivum L.) cultivars. Russ. J. Agri. Socio-Econ. Sci., 12(12) : 41-46.

[44] Richards R.A.,Rebetzke G.I.,Herwaadenet A.F. .2002 .Breeding opportunities for increasing the efficiency of water use and crop yield in temperate cereals. Crop Sci., 42 : 111-121.

[45] Shahbaz M.,Iqbal M.,Ashraf M. .2011 .Response of differently adapted populations of blue panic grass (Panicum antidotale Retz.) to water deficit conditions. J. Appl. Bot. Food Qual, 84(2) : 134-141.

[46] Shahbazi F. .2012 .A Study on the Seed Susceptibility of Wheat (Triticum aestivum L.) Cultivars to Impact Damage. J. Agril. Sci. Technol, 14(3) : 505-512.

[47] Siddique ,K.H.M. D.,Tennant M.W.,Perry R.K.,Belford R.K. .1990 .Water use and water use efficiency of old and modern wheat cultivars in a Mediterranean type environment. Aust. J. Agric. Res., 41 : 431-447.

[48] Singh G.P.,Chaudhary Y.,Rajbir S.,Tripathi S.,Agril L.). Ind. J. .2008 .Genetic analysis of moisture stress tolerance in segregating populations of bread wheat (T. , 78(10) : 848-852.

[49] Wuest S.B. and L.K.,Lutcher S.B. and L.K. .2012 .Soil water potential requirement for germination of winter wheat. Soil Sci. Soc. Amer. J., 77 : 0110.

[50] Yıldırım M.,Barutçular C.,Hossain A.,Koç M.,Dizlek H.,Akinci C.,Toptaş I.,Basdemir F.,Islam M.S.,Sabagh A. El .2018 .Assessment of the grain quality of wheat genotypes grown under multiple environments using GGE Biplot analysis. Fresenius Environ. Bull., 27(7) : 4830-4837.

[51] Zhao C.X.,Deng X.P.,Zhang S.Q.,Ye Q.,Steudle E.,Shan L. .2004 .Advances in the studies on water uptake by plant roots. Acta Bot, 46 : 505-514.

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