Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of in Vitro Produced Nili Ravi Buffalo (Bubalus Bubalis) Embryos. | [Pakistan Journal of Zoology • 2019]

Author(s):

1. Asima Azam: Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar, Peshawar, Pakistan

2. Saima Qadeer: Department of Zoology, University of Education, Jauharabad Campus, Jauharabad, Pakistan

3. Qaisar Shahzad: Buffalo Research Institute Pattoki, Kasur, Lahore, Pakistan

4. Rabea Ejaz: Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar, Peshawar, Pakistan

5. Nemat Ullah: Department of Animal Reproduction, Riphah International University, Lahore, Pakistan

6. Tasneem Akhtar: Buffalo Research Institute Pattoki, Kasur, Lahore, Pakistan

7. Shamim Akhter: Animal Physiology Laboratory, Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan

8. Asma-Ul-Husna: Animal Physiology Laboratory, Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi 46300

Abstract:

The objective of the current study was to investigate and compare the role of buffalo oviductal epithelial cell (BOEC) co-culture and conditioned culture media, on in vitro development of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Group IV: M199 alone, Group V: M199 + co-culture and Group VI: Conditioned M199 for in vitro culture (IVC). The percentage of embryos capable of crossing 8-16 cell block and reaching morula stage were higher (P < 0.05) in Group II and Group III compared to other treatments. Both treatment groups were equally effective (P > 0.05). Results also indicated that synthetic oviductal fluid (SOF) medium was found better than M199 when co-cultured and conditioned with buffalo oviductal epithelial cells for culturing buffalo embryos. Conditioned SOF is recommended to be used as IVC media in future studies as it eliminates contamination problem, remove batch to batch variation, long-lived and is readily available.

Page(s): 2007-2014

DOI: 10.17582/journal.pjz/2019.51.6.2007.2014

Published: Journal: Pakistan Journal of Zoology, Volume: 51, Issue: 6, Year: 2019

Keywords:

IVF , Oviductal cell , Coculture , Conditioned media

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