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Vigna radiata Chromium Stress-Induced Oxidative burst & its management by indigenous Plant Extracts
Author(s):
1. Ansa Javaid: School of Botany, Minhaj University,Lahore,Pakistan
2. Sundas Akhtar: School of Botany, Minhaj University,Lahore,Pakistan
3. Natasha Hassan: School of Botany, Minhaj University,Lahore,Pakistan
Abstract:
-Mung bean (Vigna radiata L. Wilczek) also known as green gram is an important edible pulse crop that are rich in protein and have a potential to fix atmospheric nitrogen. After chickpea it is an important cash crop that is cultivated on arid region of Pakistan. Unfortunately, due to various abiotic and biotic factors production of that crop is very low in Pakistan as compared to other countries. Amongst abiotic constraints accumulation of heavy metal Chromium Cr (VI) is threating food safety issue that impact on mung bean production in Pakistan. The contemporary study was conducted to ascertain the impact of Cr (VI) on the physiology, germination and growth of mung bean. In the present study the petri plate experiment was conducted in the lab. The surface sterilized seeds of mung bean were sown in different concentrations of Cr (VI) and left for 10 days. After 10 days of seeds germination different physiological attributes like total chlorophyll content (TCC) and soluble sugar (SUG) were studied. Biochemical traits i.e, Total protein content (TPC) and Catalase activity (CAT) were studied after 10 to 15 days of seed germination. Moreover, the growth parameters like germination % root and shoot length, biomass and metal accumulation were studied after 15 days of mung bean plant. Furthermore, the increasing dose of Cr (VI) exhibited deleterious effect on the root and shoot growth of mung bean while, it showed no effect on germination rate. At highest dose of Cr (VI) germination index (GI) and relative injury rate (RIR) was significantly reduced up to 57% and 0.2% respectively, over negative control. Over and above, at 1.0 to 2.0 mg kg-1 of Cr (VI) exhibited deleterious effect on both root and shoot (height and biomass). The root length and biomass was drastically reduced by 94% to 49% as compared to negative control whereas, the shoot growth (length and biomass) were gradually reduced by 87 to 61% with respect to negative control. Cr (VI) accumulation by different plant parts (root and stem) was increased with increased in heavy metal concentration (0.5-2.0 mg kg-1) in order of: root > shoot. Cr (VI) accumulation by different plant parts of mung bean plant was gradually increased in a concentration dependent manner with increased Cr (VI) concentration. Moreover, the mung bean plant is also accumulating the Cr (VI) from the soil that would be an alarming situation for the future that it could enter into our food stuff (grains, plants used as fodder crop) that pose risks and hazards to humans and animals. So far, there should be the environment friendly way to mitigate the distressing heavy metal pollution from soil and water (streams, rivers and ground water).
Page(s): 0-0
DOI: DOI not available
Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
stress , Vigna radiata , Growth , Chromium , Physiological alterations
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