Toxicological Evaluation of Tilmicosin after Intramuscular Injection in Broiler Chicken. | [Journal of Animal and Plant sciences • 2018]

Author(s):

1. MA Rasheed: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

2. M Ashraf: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

3. A Javeed: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

4. AA Anjum: Department of Microbiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

Abstract:

Cardiotoxic effects are more obvious with injectable dosage forms of tilmicosin. This study was designed to eval uate the toxicity of tilmicosin in broiler chicken after intramuscular injection. Seventy chicks were divided into seven groups. Group A was kept as control while groups B, C, D, E, F and G were administered tilmicosin at dose of 6.25, 12.5, 25, 50, 100 and 200mg/kg BW intramuscular for three days. Mortality was recorded. The birds treated with 6.25, 12.5, 25mg/kg of tilmicosin survived, while birds treated with 50, 100 and 200mg/kg died during first 30 minutes of injection. Serum samples were analyzed biochemically using spectrophotometer. A significant increase in levels of ALT, AST, total bilirubin, creatinine, blood urea nitrogen and decrease in levels of ALP, total protein and albumin was seen in groups treated with 12.5 and 25mg/kg of tilmicosin. Tilmicosin at 25mg/kg exhibited mild to severe lesions on liver, kidney and heart respectively. The birds treated with 50,100 and 200mg/kg of tilmicosin showed lesions on heart. Results were analyzed using two-way ANOVA followed by Bonferroni post analysis test. Tilmicosin elicited no significant alterations in biochemical and histopathological parameters at doses 6.25, 12.5mg/kg while mild alterations were observed at dose 25mg/kg. The 50, 100 and 200mg/kg doses of tilmicosin exhibited no change in the histopathological parameters of liver and kidney while changes were seen on heart. It is concluded that tilmicosin should be used intramuscular with care in therapy for not more than three days.

Page(s): 1678-1686

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 28, Issue: 6, Year: 2018

Keywords:

Keywords are not available for this article.

References:

[1] Altunok , V.,E.Yazar,M.Elmas,B.Tras,A.L.Bas,R.Col, 2002.Investigation of hematological and biochemical side effects of tilmicosin in healthy New Zealand rabbits,J Vet 49 68 -70

[2] Bancroft , J.D.,A . Steven, 1990.Theory and practical of histological techniques,3rd Edition 21 -106

[3] Doumas , B.,D.D. Bayse , R.J.,Carter , T.,Peters and R.,Schaffer, 1981.A candidate reference method for the determination of total protein in serum,Clin Chem 27 1642 -

[4] Er , F.,G.Altan,B.Cetin,M.Dik,E.Yazar, 2011.Assessment of the cardiotoxicity of tulathromycin in rabbits,Acta Veterinaria Hungarica 59 327 -

[5] Fit , N.,F.Chirila,G.Nadas, 2012.Haematological, biochemical and microbiological studies on dogs treated with 10% erythromycin solution,Bulletin of the University of Agricultural Sciences & Veterinary Medicine 69 91 -

[6] Gheith , I.,A.El-Mahmoudy,A.Elmajdoub,S.Awidat, 2015.Pharmacovigilance of Tilmicosin in Mice,Acta Scientiae Veterinariae 43 1318 -

[7] Jordan , W.H.,T.F.Markey,L.M.Oakley,T.L . Torrence,Nos. B-D-,B-E-, 1987.The acute dermal and inhalation toxicity and ocular and dermal irritation of tilmicosin technical, -

[8] Jordan , W.H.,R.A.Byrd,R.L.Cochrane,G.K.Hanasono,J.A.Hoyt,B.W.Main,Sarazan,Vet, 1993.A review of the toxicology of the antibiotic Micotil 300, 35 151 -

[9] Jordan , W.H., 1992.The drug tolerance study of tilmicosin administered subcutaneously to beef cattle,Am. J Vet 75 1159 -1165

[10] Kempf , L.,Reeve-JohnsonL,GesbertF,GuittetM., 1997.Efficacy of tilmicosin in the control of experimental Mycoplasma gallisepticum infection in chickens,Avian Dis 41 802 -807

[11] D.Fülöpová,P.Massányi, 2012.Effect of macrolide antibiotics on various cell cultures in vitro: 2. cell biochemistry,J microb biotech food sci. 2 1079 -

[12] Mossad , E.,A.Elkomy,M.Aboubakr,M.Morad,ArticleID, 2014.Tissue Residues, Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken,Veterinary Medicine International Volume http -

[13] Prescott , J. and D.,Baggot, 1993., 179 -204

[14] Riviere , J.E.,M.G.Papich, 2009.Veterinary Pharmacology, 868 -897

[15] Ramzan , M.,M.Ashraf,H. A.Hashmi,Z.Iqbal,A. A.Anjum, 2015.Evaluation of diclofenac sodium toxicity at different concentrations in relation to time using broiler chicken model,The J. Anim. Plant Sci 25 357 -365

[16] Said , A.A.,F.Abdel-Alim,M.Sameh,El-Nabtity , M.B.,Eldin and M.A.,Fadel, 2016.Immunological and Biochemical Profiles of Tilmicosin in Rabbits, 44 48 -55

[17] Tietz N.W.,B SaundersCo, 1999.Textbook of clinical chemistry, -

[18] Vermeulen , B.,P.D.Backer,J.P.Remon, 2002.Drug administration to poultry,Advanced Drug Delivery Reviews 54 795 -

[19] Wallhofer , H.E.,Schmidt , U.F.W.,Schmidt, 1980.Method for determination of total bilirubin and total protein,Clin Chem ACTA 105 142 -172

[20] Xie , S.,W.Fenghua,Y.Wanga,L.Zhua,Z.Donga,X.Wanga,X.Lib,W.Zhoua, 2011.Acute toxicity study of tilmicosin-loaded hydrogenated castor oil-solid lipid nanoparticles Particle and Fibre Toxicology, 8 33 -43

[21] Yazar , E.,V.Altunok,M.Elmas,B.Tras,A.L.Bas,V.Özdemir, 2001.Effect of tilmicosin on cardiac muscle and serum creatine kinases activities and serum total protein level in healthy male Balb/C miceRevue Méd, 152 881 -883

[22] Yazar , E.,Y.O.Birdane,M.Elmas,B.Tras,A.L.Bas, 2002a.Effect of tilmicosin on serum creatine kinase, creatine kinase-MB and troponin I levels in New Zeland White Rabbits,Arch. Geflu gelk. 66:1 3. -

[23] Yazar , E.,V.Altunok,M.Elmas,B.Tras,A.Bas,V.Ozdemir, 2002b.The effect of tilmicosin on cardiac superoxide dismutase and gluthatione peroxidase activities,J. Vet. Med 49 209 -

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