Author(s):
1. Samiyah Tasleem:
Department of Biotechnology, Hamdard University. Pakistan
Abstract:
The increasing resistance for antibiotics and variety of infections caused by Staphylococcus aureus has increased public health concern. There is a growing need of effective antibiotics to treat both systemic and localized staphylococcal infections. The acquiring of inducible clindamycin resistance and expression of integrons gene are well documented in Gram-negative bacteria of clinical origin, but are less documented in Gram- positive bacteria, especially of food origin. In this study, raw milk from Karachi was selected as a source to investigate this food-born pathogen. The objective of this study was to characterize phenotypic and molecular detection of inducible clindamycin resistance and investigate gation expression of of class 1 integrase gene (int1) in Staphylococcus aureus isolates from raw milk samplessold at Karachi. A total of 50 milk samples were collected and 120 isolates were selected for phenotypic identification with the help of different various morphological and biochemical tests. From 120 isolates, 50 isolates were selected for more specific analysis. D-test was performed for phenotypic detection of inducible clindamycin resistance on Mueller Hinton Agar plates. Out of 50 isolates, 8 isolates showed phenotypically inducible clindamycin resistance. DNA of these isolates was extracted to study genes expression. Polymerase chain reaction (PCR) used for the amplification of 16S rRNA, ermA, ermC, and int1 genes. The ermA gene was not amplified in any of the isolates, while ermC gene and int1 gene prevalence was 30 % and 20 %, respectively. This was the first study on S. aureus of food origin to analyze the phenomena of inducible clindamycin resistance and expression of class 1 integrase gene.
Page(s):
285-285
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
PCR
,
S aureus
,
16S rRNA
,
int1
,
inducible Clindamycin resistance
,
ermC
,
Dtest
,
ermA
References:
References are not available for this document.
Citations
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