6+ Integrated effect of Compost and Cr Reducing Bacteria on Antioxidant System and Plant Physiology of Alfalfa. | [International Journal of Agriculture and Biology • 2018]

Author(s):

1. Muhammad Usman Saleem: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan

2. Hafiz Naeem Asghar: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan

3. Zahir Ahmad Zahir: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan

4. Muhammad Shahid: Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan

Abstract:

Toxicity of chromium is highly dependent on its oxidation state. Mainly in natural ecosystem, it persists in two stable states Cr3+ and Cr6+. Among these states, Cr6+ is 100-1000 times more toxic as compared to the reduced form Cr3+. Microbes (bacteria) have the ability to reduce Cr6+ to Cr3+ and this process can be further boosted with organic matter application. Present study was designated in 2016-2017 at the Wire house of Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad to investigate the reduction potential of bacteria and their role in strengthening of plant anti-oxidant system and chlorophyll contents. Three pre-isolated and well characterized microbial strains Q5, U32 and U37 alone as well as in the presence of compost were used. Strain Q5 belongs to specie Alcaligenes, while U32 and U37 were Pseudomonas specie. It was a pot experiment and tester crop was alfalfa. Soil was Spiked with potassium dichromate salt and 24 mg kg-1 level was maintained (This was the average concentration at the fields irrigated with tannery effluent from previous study). Chlorophyll contents (a and b), antioxidant system (ascorbate peroxidase, glutathione reductase, superoxide dismutase and catalase) and physiological attributes (photosynthetic and transpiration rate, stomatal conductance and water use efficiency) were studied by following standard analytical methods and statistical procedure. Results of the study indicated that inoculation with microbial strain U32 in the presence of compost enhanced Cr6+ reduction upto 53% and grain yield 44% more over the control. Overall inoculation with U32 had improved the plant growth, physiology, anti-oxidant production, alfalfa grain yield and Cr6+ reduction was 53% more over the control.

Page(s): 2745-2752

DOI: 10.17957/IJAB/15.0829

Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 12, Year: 2018

Keywords:

Keywords are not available for this article.

References:

[1] Aebi , H., 1974.,In: Methods of Enzymatic Analysis 673 -684

[2] Ahmed,M.M.M. , M.B.,Mazen , N.A.,Nafady and O.A.,Monsef, 2017.Bioavailability of cadmium and nickel to Daucus carota L. and Corchorus olitorius L. treated by compost and microorganisms,Soil Environ 36 1 -12

[3] Ahmed , E.,H.M.Abdulla,A.H.Mohamed,A.D., 2016.Remediation and recycling of chromium from tannery wastewater using combined chemical-biological treatment system,Proc. Saf 104 1 -10

[4] Alaraidh , I.A.,A.A.Alsahli,E.S.Abdel-Razik, 2018.Alteration of antioxidant gene expression in response to heavy metal stress in Trigonella foenum-graecum L. S. Afr, 115 90 -93

[5] Ali , H.,E.Khan,M.A.Sajad, 2013.Phytoremediation of heavy metalsconcepts and applications, 91 869 -881

[6] Anonymous, 2006.Standards of medical care in diabetes,Diab. Care 29 4 -

[7] Arnon , D.I., 1949.Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris,Plant 24 1 -

[8] Azpilicueta , C.E.,M.P.Benavides,M.L.Tomaro,S.M.Gallego, 2007.Mechanism of CATA3 induction by cadmium in sunflower leaves,Plant 45 589 -595

[9] Barrera-Diaz , C.E.,L.L.Violeta,B.Bryan,Hazard, 2012.A review of chemical, electrochemical and biological methods for aqueous Cr (VI) reduction, 223 1 -12

[10] Basha , S.,Z.V.P.Murthy, 2007.Kinetic and equilibrium models for biosorption of Cr (VI) on chemically modified seaweed, Cystoseira indica, 42 1521 -1529

[11] Bates , L.S.,R.P.Waldren,I.D.Teare, 1973.Rapid determination of free proline for water-stress studies,Plant Soil 39 205 -207

[12] Bolan , N.S.,D.C.Adriano,R.Natesan, 2003.Effects of organic amendments on the reduction and phytoavailability of chromate in mineral soil,J. Environ. Qual. 32 120 -128

[13] Brose , D.A.,B.R.James, 2010.Oxidation-reduction transformations of chromium in aerobic soils and role of electron-shuttling quinones,Environ. Sci. Technol 44 9438 -9444

[14] Brown , S.L.,R.L.Chaney,Municipal,J.F.,W.A., 2000.Combining by-products to achieve specific soil amendment objectives,Soil Science Society of America 343 -360

[15] Chiu , C.C.,Lin , K.W.,Juang and D.Y.,Lee, 2009.The effectiveness of four organic amendments for decreasing resinextractable Cr(VI) in Cr(VI)-contaminated soils,J. Hazard. Mater. 161 1239 -1244

[16] Davies , F.T.,J.D.Puryear,R.J.Newton,J.N.Egilla,J.A.S.Grossi, 2002.Mycorrhizal fungi increase chromium uptake by sunflower plants: influence on tissue mineral concentration, growth, and gas exchange,J. Plant. Nutr 25 2389 -2401

[17] Debadatta , D.,M.Susmita, 2012.Simultaneous reduction of phenol and chromium from textile industry effluent using mixed culture of microorganisms,J. Environ. Res. Dev. 344 -349

[18] Dhir , B.,P.P.Saradhi,S.A.Nasim, 2009.Physiological and antioxidant responses of Salvinia natans exposed to chromium-rich wastewater,Ecotoxicol. Environ. Saf. 72 1790 -1797

[19] Etesami , H., 2018.Bacterial mediated alleviation of heavy metal stress and decreased accumulation of metals in plant tissues: mechanisms and future prospects,Ecotoxicol. Environ. Saf. 47 175 -191

[20] Fu , R.,D.Wen,X.Xia,W.Zhang,Y.Gu, 2017.Electrokinetic remediation of chromium (Cr)-contaminated soil with citric acid (CA) and polyaspartic acid (PASP) as electrolytes,Chem 316 601 -608

[21] Gamalero , E.,G.Berta,B.R.Glick, 2009.Beneficial role of plant growth promoting bacteria and arbuscular mycorrhizal fungi on plant responses to heavy metal stress,Can. J. Microbiol. 55 501 -514

[22] Gheju , M.,I.Balcu,M.Ciopec, 2009.Analysis of hexavalent chromium uptake by plants in polluted soils,Ovidius Uni. Ann. Chem 20 127 -131

[23] Gu , B.,J. Chen, 2003.Enhanced microbial reduction of Cr(VI) and U(VI) by different natural organic matter fractions,Geochim 67 3575 -3582

[24] Gutierrez-Corona , J.F.,P.Romo-Rodríguez,F.Santos-Escobar,A.E.Espino-Saldana,H.Hernandez-Escoto, 2016.Microbial interactions with chromium: basic biological processes and applications in environmental biotechnology,World J. Microbiol 32 191 -

[25] Hao , X.H.,S.L.Liu,J.S.Wu,R.G.Hu,C.L.Tong,Y.Y.Su, 2008.Effect of long-term application of inorganic fertilizer and organic amendments on soil organic matter and microbial biomass in three subtropical paddy soils,Nutr 81 17 -24

[26] Hossain , Z. and S.,Komatsu, 2013.Contribution of proteomic studies towards understanding plant heavy metal stress response,Front. Plant Sci. 3 310 -

[27] Humphries , E.C.,Paech , K.,M.V.Tracy, 1956.Modern Methods of Plant Analysis, 481 -

[28] Islam , F.,T.Yasmeen,Q.Ali,S.Ali,M.S.Arif,S.Hussain,H.Rizvi, 2014.Influence of Pseudomonas aeruginosa as PGPR on oxidative stress tolerance in wheat under Zn stress,Ecotoxicol. Environ. Saf. 104 285 -293

[29] Islam , H.S., 2016.Bioremediation of chromium,Res. J. Chem 20 1 -

[30] Iyer , V.G. and N.E.,Mastorakis, 2010.Unsafe chromium and its Environmental Health Effects of Orissa Chromate Mines,Proc. Int. Conf. Universitatea Politechnica 111 -122

[31] Jambunathan , N., 2010.Determination and detection of reactive oxygen species (ROS), lipid peroxidation and electrolyte leakage in plants,In: Plant Stress Tolerance 291 -297

[32] Licina , V., S.,Antic-Mladenovic and M.,Kresovic,Fragaria, 2007.) after the amelioration of coalmine tailing soils with different organomineral amendments,Arch. Agron. Soil Sci. 53 39 -48

[33] Lie , D.,J.Zou,M.Wang,W.Jiang, 2008.Hexavalent chromium uptake and its effects on mineral uptake, antioxidant defense system and photosynthesis in Amaranthus viridis L. Bioresour, 99 262 -

[34] Liu , Y.,R.Jin,T.Tian,J.Zhou, 2017.Effects of hexavalent chromium on performance, extracellular polymeric substances and microbial community structure of anaerobic activated sludge in a sequencing batch reactor,J. Chem. Technol. Biotechnol 92 2719 -2730

[35] Malaviya , P.,A.Singh, 2016.Bioremediation of chromium solutions and chromium containing wastewaters,Crit. Rev. Microbiol. 42 607 -633

[36] Maleki , M.,M.Ghorbanpour,K.Kariman, 2017.Physiological and antioxidative responses of medicinal plants exposed to heavy metals stress,Plant Gene 11 247 -254

[37] Merdy , P.,Y.Lucas, 2009.Cu and Cr interactions with soil: sorption experiments and modelling,Colloids Surf. A. 347 192 -199

[38] Monte , M.C.,E.Fuente,A.Blanco,C.Negro, 2009.Waste management from pulp and paper production in the European Union, 29 293 -308

[39] Moodie,C.D. , H.W.,Smith and R.A.,McCreery, 1959.Organic matter determination,In: Laboratory Manual for Soil Fertility 31 -39

[40] Mulligan , C.N.,R.N.Yong,B.F.Gibbs, 2001.Remediation technologies for metal-contaminated soils and groundwater: an evaluation,Eng 60 193 -207

[41] Nakano , Y.,K.Asada, 1981.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts,Plant Cell Physiol 22 867 -880

[42] Oliveira , H., 2012.Chromium as an environmental pollutant: insights on induced plant toxicity,J. Bot. 1 -8

[43] Panda , S. K. and S.,Choudhury, 2005.Chromium stress in plants,Braz. J. Plant Physiol 17 95 -102

[44] Parameswari , E.,A.Lakshmanan,T.Thilagavathi, 2009.Effect of pretreatment of blue green algal biomass on bio-adsorption of chromium and nickel,J. Algal Biomass Utili. 1 9 -17

[45] Pereyra , M.A.,C.A.Zalazar,C.A.Barassi, 2006.Root phospholipids in Azospirillum-inoculated wheat seedlings exposed to water stress,Plant 44 873 -879

[46] Prasad , M.N.V.,M.Greger,T.Landberg, 2001.bark removes toxic elements from solution: corroboration from toxicity bioassay using Salix viminalis L. in hydroponic system,Int. J. Phytorem. 3 289 -300

[47] Pugazhenti , G., S.,Sachan , N.,Kishore and A.,Kumar, 2005.Separation of chromium (VI) using modified ultra-filtration charged carbon membrane and its mathematical modelling,J. Member. Sci. 254 229 -239

[48] Rajkumar , M., S.,Sandhya , M.N.V.,Prasad and H.,Freitas, 2012.Perspectives of plant-associated microbes in heavy metal phytoremediation,Biotechnol 30 1562 -1574

[49] Rashid , H.,J.Takemura,A.M.Farooqi, 2012.Investigation of subsurface contamination due to chromium from tannery effluent in Kasur District of Pakistan,J. Environ. Sci. Eng 1 1007 -1024

[50] Saranraj , P.,D.Sujitha, 2013.Microbial bioremediation of chromium in tannery effluent: a review,Int. J. Microbiol. Res. 4 305 -320

[51] Sathish , T.,N.V.Vinithkumar,G.Dharani,R.Kirubagaran, 2015.Efficacy of mangrove leaf powder for bioremediation of chromium (VI) from aqueous solutions: kinetic and thermodynamic evaluation,Appl. Water Sci. 5 153 -160

[52] Shahid , M., S.,Shamshad , M.,Rafiq , S.,Khalid , I. Bibi, N.K.,Niazi , C.,Dumat and M.I. Rashid, 2017.Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: A review,Chemosphere 178 513 -533

[53] Shanker , A.K.,R.Sudhagar,G.Pathmanabhan, 2003.Phytochelatin SH and antioxidative response of sunflower as affected by chromium speciation,In: 2nd Int. Congr. Plant Physiol. Sus. Plant Prod. Chang. Environ -

[54] Shanker , A.K.,C.Cervantes,H.Loza-Tavera,S.Avudainayagam, 2005.Chromium toxicity in plants,Environ. Int. 31 739 -753

[55] Sharma , P.,H.S.Gujral,B.Singh, 2012.Antioxidant activity of barley as affected by extrusion cooking,Food Chem 131 1406 -1413

[56] Sharma , S.,A.Adholeya, 2011.Detoxification and accumulation of chromium from tannery effluent and spent chrome effluent by Paecilomyces lilacinus fungi,Int. Biodeter. Biodegrad. 65 309 -317

[57] Tangahu , B.V.,S.R.S.Abdullah,H.Basri,M.Idris,N.Anuar,M.Mukhlisin, 2011.A review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation,Int. J. Chem 201 1 -31

[58] Trebien , D.O.P.,M.J.Tedesco,C.A.Bissani,F.A.O.Camargo, 2011.Environmental factors affecting chromiummanganese oxidation-reduction reactions in soil,Pedosphere 21 84 -89

[59] U.S. SalinityLab,USGovt, 1954.Diagnosis and improvement of saline and alkali soils,In: USDA Handbook No. 60 -

[60] Wang , C.,H.Deng,F.Zhao, 2016.The remediation of chromium (VI)- contaminated soils using microbial fuel cells,Soil Sediment. Contam. Int. J. 25 1 -12

[61] Watanabe , F.S. and S.R.,Olsen, 1965.Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from the soil,Soil Sci. Soc. Am. J. 29 677 -678

[62] Yadav , S.K., 2010.Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S. Afr, 76 167 -179

[63] Yen , H.W.,P.W.Chen,C.Y.Hsu,L.Lee, 2017.The use of autotrophic Chlorella vulgaris in chromium (VI) reduction under different reduction conditions,J. Taiw. Ins. Chem 74 1 -6

[64] Yu , B.,H.Zhang,G.Li,Z.Wu, 2014.Remediation of chromium-slag leakage with electricity cogeneration via a urea-Cr (VI) cell,Sci. Rep 4 5860 -

[65] Zhou , Z.S.,K.Guo,A.A.Elbaz,Z.M.Yang, 2009.Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa,Environ. Exp. Bot. 65 27 -34

[66] 2018.,(Received 02 February 2018; Accepted 24 July -

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