Abstract:
Medicinal plants, with their rich repertoire of bioactive secondary metabolites, serve as vital sources of natural products with therapeutic potential. Monotheca buxifolia (Falc.) A. DC., traditionally used against kidney, liver, and digestive disorders. However, a comprehensive scirntific validation ot its phytochemical composition and multifaceted phramcological properties remain linited. This study aims to bridge this gap by systematically investigating M. buxifolia leaves and stems extracts and fractions.The crude methanol extracts were subjected to vioactivity-guided fractionation using column chromatography. The compounds were characterized by nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HR-MS). A comprehensive phytochemical and pharmacological evalution was undertaken, cncompassing the quantification of total phenolic and flavonoid contents. Antioxidant capacity was assessed by various assays: total antiocidant capacity (TAC), total reducing power (TRP), and radical scavenginh (DPPH, and ABTS). The therapeutic potential was further evaluated through enzyme inhibition assays targeting key metabolic enzymes (a-amylase, urease, and lipase). Antimicrobial efficacy was tested against a panel of bacterial and fungal strains, and toxicity was evaluated using a brine shrimp letality assay. Phytochemical analysis led to the identification of three principle compounds such as uercetin (MM-1), 2-hydroxybenzoic acid ( Salicylic acid; MM-2), quercetin-3-O-rutinoside (Rutin; MM-6). These showed significant dose-dependent antioxidant activities, with quercetin exhibiting the most potent effects, nothably in reducing power (86.9 µg AAE/mg) and metal chelation (83.8%). The extracts and fractions showed remarkable enzyme inhibition, particularly against urease (69.8-80.6%) and lipase (64.0-78.9%) suggesting potential for managinf digestive and metabolic diorders. Antimicrobial assays revealed broad-spectrum activity with pronounced inhibition zones against Fusarium solani (18 mm) and Staphylococcus aureus (15 mm). The study provide a robust scientific foundation for ethnopharmacological importance of M. buxifolia. The isolated flavonoids and phenolics compounds are established as potent antioxidant, antimicrobial, and enzyme inhibitory agents. These findings underscore the potential of M. buxifolia as a promising source for developing natural product-based the natural product-based therapeutics for infections, metabolic, and oxidative stress-related diseases.
Page(s):
50-50
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
antimicrobial activity
,
Antioxidant activity
,
enzyme inhibition
,
natural products
,
Monothea buxifolia