Variation in bioactive principles of Artemisia amygdalina Decne. in wild and tissue culture regenerants. | [Pakistan Journal of Pharmaceutical Sciences • 2013]

Author(s):

1. Rafia Rasool: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India

2. Bashir Ahmad Ganai: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India

3. Seema Akbar: Regional Research Institute of Unani Medicine, Kashmir University Campus, Srinagar, Jammu and Kashmir, India

4. Azra Nahaid Kamili: Department of Environmental Sciences, Centre of Research for Development, University of Kashmir, Srinagar, J&K, India

5. Muhammad Younus Dar: Regional Research Institute of Unani Medicine, Kashmir University Campus, Srinagar, Jammu and Kashmir, India

6. Akbar Masood: Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India

Abstract:

Wild and tissue culture raised regenerants of Artemisia amygdalina, a critically endangered and endemic plant of Kashmir and North West Frontier Provinces of Pakistan were screened for the amount of bioactive principles and in particular antimalarial compound artemesinin. Phytochemical screening of extracts revealed the presence of terpenes, alkaloids, phenolics, tannins (polyphenolics), cardiac glycosides and steroids in wild (aerial, inflorescence) and tissue culture regenerants (in vitro grown plant, callus and green house acclimatized plants). HPLC of Artemisia amygdalinarevealed the presence of artemesinin in petroleum ether extracts of wild aerial part, tissue culture raised plant and green house acclimatized plants. Acetonitrile and water in 70:30 ratios at flow rate of 1ml/min was standardised as mobile phase. Retention time for standard chromatogram was 6.7. Wild inflorescences and callus does not produce artemesinin. This is the first report of phytochemical screening and artemesinin estimation of wild and tissue culture raised regenerants of Artemisia amygdalina.

Page(s): 623-628

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 26, Issue: 3, Year: 2013

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