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Trial for Preparation and Evaluation of Autogenous Killed Vaccine Against Some Locally Isolated Strainsof Salmonella enterica from Chickens in Egypt
Author(s)
Nagwa S Rabie Poultry Diseases Department, National Research Centre,P.O. 12622, Giza,Egypt
Hazem M Ibrahim Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre,,Egypt
Aly M Ghetas Department, National Research Centre,P.O. 12622, Giza,Egypt
MM Abdelbaki Department, National Research Centre,P.O. 12622, Giza,Egypt
Hanaa S Fedawy Department, National Research Centre,P.O. 12622, Giza,Egypt
Dalia M Sedeek Department, National Research Centre,P.O. 12622, Giza,Egypt
MA Bosila Department, National Research Centre,P.O. 12622, Giza,Egypt
Kh M Elbayoumi Department, National Research Centre,P.O. 12622, Giza,Egypt
AA Samy Department, National Research Centre,P.O. 12622, Giza,Egypt
Abstract
A trivalent killed Salmonella vaccine was developed and its efficacy was evaluated in specific pathogen free (SPF) chickens. One hundred and forty SPF chickens divided to two groups were used to evaluate the efficacy of our vaccine. Vaccinated chickens were primed with the prepared vaccine at the age of two weeks and were boosted three weeks later comparing to unvaccinated chickens. All chickens were divided into subgroups which challenged orally with 1mL (5x107 CFU/mL) of Salmonella Typhimurium (ST), Salmonella Montevideo (SM), or Salmonella Blegdam (SB) strains, three weeks post boosting of the vaccine. The protection rate was evaluated based on the clinical signs, the mortality, and bacterial fecal shedding. Moreover, we used ELISA technique to evaluate the humoral immune response against Salmonella strains. Our trivalent killed Salmonella vaccine elicited 85% protection rate in challenge test with reduction of bacterial fecal shedding.
Publication Details
Page(s) 456-461
DOI 10.47278/journal.ijvs/2022.209
Published Journal: International Journal of Veterinary Science, Volume: 12, Issue: 3, Year: 2023
Keywords
Chicken Salmonella typhimurium vaccine Salmonella Montevideo Salmonella Blegdam Montanide ISA 70
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