Article Detail — Pakistan Science Abstracts

Choose Theme

Theme 1 — Green & Gold

No Detail Found!
Xylanase Production from Bacillus Subtilis in Submerged Fermentation using Box-behnken Design.
Author(s)
Sobia Naz Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Muhammad Irfan Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Muhammad Umar Farooq Food Science Department, Muhammad Nawaz Sharif University of Agriculture, Multan Pakistan
Abstract
In this study, an attempt was made to optimize the nutritional conditions for xylanase production by Bacillus subtilis in submerge fermentation process using agricultural waste like corn cobs as substrate. Three variables with three levels such as corn cobs loading (1, 3, 5, % w/v), peptone (0.05, 0.275, 0.5%) and KH2PO4 (0.1, 0.3, 0.5%) were optimized through Box-Bhenken design of response surface methodology. It was revealed that the maximum yield of xylanase (295 U/ml) was achieved with 3% corn cobs as substrate, 0.05 (%) peptone, and 0.5 (%) KH2PO4. Analysis of variance reveals that the proposed model was significant having an F value of 188.77 and its corresponding p values 0.000 and, P>F<0.0001 shows the model's accuracy. Higher R2 values (98.93) of the model depicted that only 1.07% variations could not be explained by the model. Findings of this study could be utilized for industrial exploitation of the enzyme.
Publication Details
Page(s) 151-156
DOI DOI not available
Published Journal: Pakistan Journal of Biotechnology, Volume: 14, Issue: 2, Year: 2017
Keywords
Keywords are not available for this article.
References
Aarti , C.,M.V.Arasu,P.Agastian, 2015.Lignin degradation: A microbial approach,S. Indian J. Biol. Sci 1 119 -27
Bernier , R.,M.Desrochers,L.Jurasek,M.G.Paice, 1983.Isolation and characterization of Xylanase from Bacillus subtilis,Appl. Env. Microbiol 46 511 -514
Bas , D.,I H.Boyaci, 2007.Modeling and optimization I: usability of response surface Methodology,J Food Eng 78 836 -845
Battan , B.,J. Sharma , S. S.,Dhiman , and R.C.,Kuhad, 2007.Enhanced production of cellulose-free Thermo stable by Bacillus pumilus ASH and its potential application in paper Industry,Enzyme Microb Technol 41 733 -739
Belancic , A.,J.Scarpa,A.Peirano,R.Diaz,J.Steiner,J.Eyzayuirre, 1995.Penicillium Purpurogenum produces several xylenes: purification and properties of two of the Enzymes,J Biotechnol 41 71 -79
Biely , P.,H.Schneider, 1985.Acetyl xylan esterases in fungal cellulolytic systems,FEBS Lett 186 80 -84
Bilgrami , K.S.,A K.Pandy, 1992., 149 -165
Beg , Q.K.,M.Kapoor,L.Mahajan,G.S.Hoondal, 2001.Microbial xylanases and their Industrial applications; a review,Appl. Microb. Biotechnol 56 326 -338
Chakrit , T.,L.K.Khin,R.Khanok, 2006.Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column,Process Biochem 41 2441 -2445
Chaturvedi , S.,K.U.Kohli,S.Rajni,S.M.P.Khurana, 2015.Statistical Optimization of Medium Composition for Xylanase Production by Solid State Fermentation Using Agro-residues,Ameri. J. Microbiol Res 3 85 -92
Coman , G., 2011.- 137 Using response surface methodology and central composite design,Ann Microbiol 61 773 -779
Demain , A. L., 1971.Overproduction of microbial metabolites and enzymes due to alteration of Regulation,Biochem. Eng 1 42 -113
Dey , D.,J.Hinge,A.,M.Rao, 1992.Purification and properties of extracellular Endoxylanases from alkalophilic thermophilic Bacillus Sp, 38 436 -442
De Souza , D. F.,C.G.M. de Souza , and R.M.,Peralta, 2001.Effect of easily metabolizable sugars in the production of xylanase by Aspergillus tamarii in solid-state fermentation,Process Biochem 36 835 -838
Elegir , G.,M.Sykes, 1995.Differential and synergistic action of Streptomyces Endoxylanases in prebleaching of Kraft pulp,Enz. Microb. Technol 17 954 -959
Frost , G.M.,and D.A.Moss, 1987.Production of enzymes by fermentation,Biotechnol 65 -211
Garg , N.,K K. Mahatma,A.Kumar,BiotechnologicalAspects Lambert Academic Publishing,AG & Co. KG, Germany, 2010.and, -
Gouda , M.K., 2000.Purification and partial characterization of cellulose free xylanase produced in solid state and submerged fermentation by Aspergillus tamarii,Advances Food Sci 22 31 -37
Gomes , D J.,J.Gomes,W.Stiener, 1994.Production of highly thermo stable xylanase by a wild strain of thermophilic fungus Thermoascus aurantiacus and partial characterization of the enzyme,J Biotechnol 37 11 -22
Gessesse , A.,G., 1999.Mamo, High-level xylanase production by an alkaliphilic Bacillus sp. using solid-state fermentation,Enzyme Microb. Technol 25 68 -72
Haltrich , D.,B.Nidetzky,K.D.Kulbe,W.Steiner,S.Zupanic, 1996.,Bioresour Technol 58 137 -161
Hoq , M.M.,C.Hempel,W.D.Deckwer, 1994.Cellulose free xylanase by Thermomyces lanuginose RT9: effects of aeration, agitation and medium components on production,J Biotechnol 37 49 -58
Ho , L.H and L.H.,Heng, 2015.Xylanase Production by Bacillus subtilis in Cost-Effective medium using soybean hull as part of medium composition under submerged fermentation (SmF) and solid-state fermentation (SsF),J Biodiversity. Biopros. Dev 2 -
Irfan , M.,U.Asghar,M.Nadeem,R.Nelofer,Q.Syed, 2016.Optimization of process parameters for xylanase production by Bacillus sp,in submerged fermentation J. Rad Res Appl Sci 9 139 -147
Irfan , M.,M.Nadeem,Q.Syed,S.Baig,American-EurasianJ. Agric.,Environ, 2012.Effect of Medium Composition on Xylanase Production by Bacillus subtilis using Various Agricultural Wastes, 12 561 -565
Khosarvi-Darani , K.,D.Karamad, 2016.Solid state cultivation and application of xylanase,Pak. J. Biotechnol 13 147 -156
Kulkarni , N.,M.Rao, 1996.Applications of xylanase from alkallophilic thermophilic Bacillus sp. BCIM 59 in biobleaching of bagasse pulp,J Biotechnol 51 73 -167
Lei , X.,L.Lin,K.Li, 2008.Effect of xylanase pretreatment on wood chips on fiber separation in the CTMB refining process,Bioresources 3 801 -815
MorikawaM., 2006.Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species,J Biosci. Bioengin 101 1 -8
Myers , R.H.,D.C.Montgomery, 1995.Response surface methodology: Process and product Optimization using designed experiments (Wiley International Publication, 118 -8
Nagar , S.,R.K.Jain,V.V.Thakur,V.K.Gupta, 2013.Biotechnological application of Cellulose poor and alkali stable xylanase by Bacillus pumilus SV-85S in bio-bleaching,Biotech 3 277 -85
Pandey , A,S.Benjamin,C. R.Soccol,P.Nigham,N.Krieqer,V.T.Soccol, 1999.The realm of microbial lipases in biotechnology,Biotechnol. Appl. Biochem 29 119 -31
Polizeli , M.L.T.M.,A.C.S.Rizzatti,R.Monti,H.F.Terenzi,J.A.Jorge,D.S.Amorim, 2005.Xylanases from fungi: Properties and industrial applications,Appl Microbiol Biotechnol 67 577 -591
Sharma , H.,Nauni-Solan, 1998.Optimization of extracellular xylanase production by Bacillus macerans in Solid state fermentation of apple pomace,MSc thesis -
Techapun C.,T.Charoenrat,N.Poosaran,M.Watanabe,K.Sasaki,J BiosciBioeng, 2002.Thermo stable and Alkaline-tolerant cellulose-free xylanase produced by thermo tolerant Streptomyces sp Ab 06, 4 431 -433
Veluz , G.,K.Taksuo,M.Hiroshi,F.Yusaku, 1999.Screening Rhizopus sp,J. Faculty Agri 43 419 -423
Walia , A.,P.Mehta,A.Chauhan,K.C.Shirkot, 2013.Optimization of cellulose-free xylanase Production by alkallophilic cellulosic microbium sp. CKMX1 in solid-state Fermentation of apple pomace using central composite design and response surface methodology,Ann Microbiol 63 187 -198
Xiong , Y.H.,J.Z.Liu,H.Y.Song,L.N.Ji, 2004.Enhanced production of extracellular ribonuclease from Aspergillus niger by optimization of culture conditions using response surface methodology,Biochem. Eng. J 21 27 -32
Citations
Citations are not available for this document.
0

Citations

0

Downloads

55

Views

Copyright ©  PASTIC National Center, Islamabad www.pastic.gov.pk