Abstract:
One basic polysaccharide that is frequently used in industrial settings is starch, which frequently needs to be hydrolyzed by a-amylase to produce simpler sugars. The search for sustainable and affordable enzyme production methods has prompted researchers to investigate agro-waste substrates as potential substitutes for synthetic media. Microbial strains, namely bacterial strainEP3(Bacillus Lenchiforma) and fungal strains As per gillus Niger and Penicillium, were cultivated on agro-waste-derived media made of potato, mango, and banana peels in order to create a-amylase in this study. These substrates were included in a starch-based fermentation medium that was enhanced with peptone, MgSO4, KH2PO4, yeast extract, and sun-dried and crushed. After being aseptically sterilized and inoculated, the media was incubated at regulated temperatures. By adjusting the pH (6, 7, and 8) and the fermentation length (12, 24, and 48 hours), the synthesis of enzymes was maximized. Following incubation, the culture filtrates were collected, and the DNS method was used to determine absorbance at 546 nm and test for a-amylase activity. Mango peel was found to be the most efficient substrate, and the EP3 bacterial strain showed the highest level of enzyme activity at neutral pH (7) and 24 hours. The potential of agro-waste, especially fruit peels, as affordable and environmentally beneficial substrates for industrial enzyme synthesis is highlighted by this study. With potential uses in the food, textile, pharmaceutical, and biofuel sectors, the discoveries further the development of sustainable bioprocessing technology.
Page(s):
119-119
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025