In present research the antagonistic activity of human bacterial pathogens viz., Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumonia, Staphylococcus aureus, Serratia marcescens, Escherichia coli and Pseudomonas aeruginosa were analysed. Significant amount of lactic acid production was shown by S. pyogenes, S. epidermidis and P. aeruginosa (63 mg/mL, 54 mg/mL, and 54 mg/mL), while S. pyogenes and S. epidermidis also produced significant quantity of hydrogen peroxide (1.70 mg/mL and 1.605 mg/mL). An impressive diversity of spots of chemical constituents was also obtained through thin layer chromatography (TLC) from broth culture of bacterial strains. The antagonistic activity may be indicated the potency of lactic acid and hydrogen peroxide production to inhibit the microbes.