Activity of Thuja (Thuja orientalis) Alcoholic Extract in Inhibition Aspergillus flavus Growth and Detoxification of Aflatoxin B1 in Contaminated Corn Seeds. | [Pakistan Journal of Biotechnology • 2017]

Author(s):

1. Amna M. Ali: Department of Sciences, College of Basic Education, Al-Mustansiriyah University, Iraq

Abstract:

The study was conducted to evaluate the activity of Thuja alcoholic extract to inhibit Aspergillus flavus growth and detoxify the aflatoxin B1 that produced. The results showed high activity of Thuja extract in inhibition of A. flavus growth on culture media that attained to 70 - 25, 85 - 40, 100% with the concentrations 0.2, 0.5, and 1% respectively. The activity of the extract in A. flavus growth inhibition was found associated with high activity in detoxification of afla B1 from contaminated corn seeds. The treatment of contaminated corn seeds with 2% of Thuja extract induced high reduction in afla B concentration. The afla B1 concentration was found to be 752.40 ng/g in treated corn seeds compared with 2620.20 ng/g in control representing 71.35% reduction in the first month reached to 100% in the second month.

Page(s): 503-506

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 14, Issue: 3, Year: 2017

Keywords:

Keywords are not available for this article.

References:

[1] Abbas H.K. , R.D. cartwright, G.L. Windhom,C.J.Mirotcha, 2000.The presence of mycatoxins and fungi in rice and corn in the southern untied state,Bull. Ins. Compr. Agri. Sci. Kunki Univ 8 21 -24

[2] Abbas , H.K.,W.T.Shier, 2009.Mycatoxin contamination of agricultural products in the southern united state and approaches to reducing in from preharvest to final food products. Mycatoxin prevention and control in agriculture,ACS Symposium series 1031 37 -58

[3] Abbas , H.K.,C.J.Miracha,R.A.Meronuck,J.D.Pokorny,S.L.Gould,T.Kommedahl,FusariumSPP, 1988.Associated with infected ears of corn in minaesota,Appl. Envir. Micro 54 1930 -1933

[4] Balzer , I., C.,Bogdanic and S.,Pepeljnjak, 1978.Rapid thin layer chromatographic method for determining aflatoxin B1, Ochratoxin A and Zearalemane in corn,Journal at the Association of official Analytical chemists 61 584 -585

[5] Caprita , A.,R.Caprita,C.Cozmiuc,B.Maranescy,H.Sarandan, 2007.Simultaneous determination of mycotoxin (ochrotoxin A and Deoxynivalenol) in biological samples,Journal of Agroalementary Processes and Tehcnology 13 353 -358

[6] Cowman , M.M., 1999.Plant products as antimicrobial agents American Society for Microbiology,Clinical Microbiology Review 12 564 -582

[7] Coker , R.D., K.,Jewer , B.D.,Sones, 1985.The destruction of Aflaloxin by ammonia,Practical Possibilities. Tropical Science 25 139 -154

[8] Christensen , C.,H.H. Kaufman, 1974.Storage of cereal grains and other products,St.paul. American Association of cereal Chemists 158-192 -

[9] Farage , R.S.,Z.Y.Daw,F.M.Hewed,G.S.EL-Bacoty, 1989.Antimicrabial activity of some Egyptian Spice essential oils,Food Prot 52 665 -667

[10] Frayssinet , C.,F.C.Lafarge, 1992.Effect of ammoniation on the carcenogenicity of aflatoxin contaminationgroundnut oil cakes: Long -term feeding study in the rat,Food Additives and Contaminants 7 63 -68

[11] Lussenhop , J. and D.T.,Wicklow, 1990.Nitidulid beetles (Nitidulidae. Coleoptera) as vectors of Aspergillus flavus in poreharvest maize,Transaction of the Mycological Society of Japan 31 63 -74

[12] Hajare , S.S., S.H.,Hajare and A.,Shrma, 2005.Aflatoxin in activation using aqueous extract of Ajwan (Trachyspermumammi) seeds,Journal of Food Science 70 29 -34

[13] Halima Z. Hussien, 2017.Detection of trichothecene of Fusarium solani isolates by using HPLC in melon plants,Pak. J. Biotechnol 14 213 -215

[14] Halima Z. Hussien , luma K.,Bandr and F.M.,Alkhilani, 2017.Testing the Efficiency of Probiotic in Reducing the Growth of Aspergillus flavus in Corn Stored and its Effect on Performance pf Broiler,RJPBCS 179 -

[15] Harborne , J.B., 1973.Phytochemical methods. A guide to modern technique analysis,Champmon 278 -

[16] Mukun , H.A.,S.T.Amjorin,B.Moronfaye,F. O.Adejo,O.A.Afolabi,G.Fagbayibo,B. O.Balogun,A.A.Surajudeen, 2010.Fungal and aflatoxin contamination of some human food commodities in Nigeria African food sci, 4 127 -135

[17] Mucphy , P.S.,K.Ramalakshmi,P.Sriniras, 2009.Fungitoxic activity of Indian Borage (Plectronthusam bionicus) volatiles,Food Chemistry 114 1014 -1018

[18] Paranagama , P.A.,K.H.T., 2003.Abey sekera, K.P. Abeywickrama and l. nugaliyadde, Fungicidal and anti-aflatoxigenic effects of the essential oil of cymbopogon citrates (DC) staps, (Lemongrass) against Aspergillus flavus Link. Isolated from stored rice,Letters in Applied Microbiology 37 85 -90

[19] Samdosskumar , R.,M.Karthikeyan,S.Mathiyazhagan,M.Mohankumas,G.Chandrasekar,R.Vetazhan, 2007.Inhibition of Aspergillus flavus growth and detoxification of aflatoxinB1 by the medicinal plant zimmu (Allium sativum L.x Allium cepa L.),World Journal of Microbiology and Biotechnology 23 1007 -1014

[20] Sapacota , O.,R.Islam,A. K.Mehdi, 2005.Efficacy of Andrographis paniculata in ameliorating aflatoxicosis in broilers,Indian Neterinary Journal 92 529 -532

[21] Shearer , J.F.,L.E.Sweats,N.K.Baker,L. H.Tiffony, 1992.A study of Aspergillus flavus parasiticus in Iowa crap fields from 1988- 1990,Plant Disease 76 88 -96

[22] Smart , M.G.,D.T.Wicklow,R.W.Caldwell, 1990.Pathogenesis in Aspergillus ear rot of maize: Light microscopy of fungal spread from wounds,Phytopathology 80 1281 -1294

[23] Zuber , M.S.,E.B.Lillehaj, 1993.: Biocontrol of plant diseases, 86 102 -

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