Green Biotechnology for Climate Resilience: Engineering Low- Viscosity Oils in Brassica juncea for Sustainable Biofuels | [1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025 • 2025]

Author(s):

1. Iqbal Munir: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

2. Ijaz Naeem: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

3. Timothy P. Durrett: Department of Biochemistry and Molecular Biophysics, Kansas State University,Manhattan, KS 66506,USA

4. Aqib Iqbal: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

5. Waqar Ali: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

6. Hamza Iqbal: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

7. Maaz Iqbal: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

8. Umair Munir: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

9. Fatima Tu Zuhra: Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar-,,Pakistan

Abstract:

Climate change remains the most pressing global challenge of the 21st century, with transport and fossil-fuel-driven machinery contributing nearly 25% of total energy related greenhouse gas emissions worldwide. Rising temperatures, unpredictable weather extremes, and resource depletion underscore the urgent need for sustainable and renewable energy alternatives. Among the many approaches, green biotechnology provides powerful tools to engineer crops for biofuel production, thereby reducing reliance on fossil fuels and mitigating environmental impacts. Brassica juncea oil represents a promising biofuel feedstock; however, its practical use is constrained by high viscosity, low volatility, and poor performance under cold conditions. To address these limitations, our research focused on metabolic engineering of B. juncea by introducing the Euonymus alatus diacylglycerol acetyltransferase (EaDacT) gene, responsible for synthesizing low-viscosity acetyl triacylglycerols (acTAGs). Through an optimized Agrobacterium-mediated transformation approach, transgenic plants were developed, and subsequent strategies involving DGAT/PDAT gene silencing combined with EaDacT overexpression successfully yielded seed oils enriched with acTAGs and reduced viscosity. This work exemplifies how recent advances in green biotechnology can contribute to climate resilience by developing renewable, plant-based energy solutions, reducing greenhouse gas emissions, and providing sustainable alternatives to fossil fuels for transport and machinery.

Page(s): 41-41

DOI: DOI not available

Published: Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025

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