Abstract:
Bacterial endophytes are a key part of the plant micro biome that influence developmental processes via different mechanisms. One of the known mechanisms is the production of exo polysaccharides (EPS) that are responsible for the response towards stresses and plant-bacterial interactions. In the current experiment, we isolated 42 strains of bacterial endophytes from the sterilized parts of Moringa oleifera plant and screened them to produce EPS. Out of the42, only three isolates; KR-27, KL-4, and KS-7 produced a significant number of EPS. All these isolates showed in vitro antagonistic activities againstphytopathogenic fungi and bacteria. These antagonistic endophytic isolates, KR-27, KL-4, and KS-7 were identified as Serratia marcescens, Klebsiellaaerogenes, and Lelliottia amnigena, respectively based on 16 s RNA generibotyping. The selected bacterial strains were screened out for phyto hormones and secondary metabolites production and all the identified strains produced significant amount of various phyto hormones such as in dole acetic acid (IAA), gibberellic acid (GA3), and salicylic acid (SA). In addition, the identified strains produced antioxidant compounds like side rophores, phenolics, and flavonoids.The phosphate solubilization ability of all the strains was ascertained and found highest for KR-27 (30.46±0.82 µg/mL), followed by KS-7 (18.30±0.54 µg/mL)and KL-4 (17.36±0.68 µg/mL). Unlike previous research, which primarily focused on the nutritional and medicinal properties of M. oleifera, this research uncovers the new aspect by exploring the biotechnologically important bacterial flora of this miracle tree. In conclusion we isolated three novel bacterial endo phytic strains with the ability to produce bioactive compounds for plant and antimicrobial activity against phyto pathogens for the first time which can replace the synthetic fertilizers and pesticides in future sustainable agriculture practices.
Page(s):
44-44
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
antimicrobial activity
,
bioactive compounds
,
Moringa oleifera
,
endophytic bacteria
,
exopolysaccharides