Abstract:
Diindolylmethane is a bioactive metabolite found in cruciferous vegetable and has anticancer potential. The major challenge to use Diindolylmethane for pharmaceutical application is stability and sensitive to the environment. This study aimed to develop the microgel of whey protein concentrate (WPC) to microencapsulate the Diindolylmethane (DIM) Solutions with different ratios of Diindolylmethane to whey protein (1:12, 1:6, 1:4, 1:3, w/w) with constant whey protein concentrate level at 12% (w/v).The samples were heated to 85°C for 30min and then treated with ultrasound for 15min or 30min, respectively. Changes in zeta potential, particle size, and rheological property were studied. Results showed that ultrasound treatment significantly reduced particle size of samples 1:12 (456.08±24.87 nm), 1:6 (537.25±29.55 nm), 1:4 (565.73±10.20 nm), and 1:3 (580.44 ±18.29 nm) to 285.67±3.08 nm, 340.03±10.52 nm, 391.47±5.44 nm, and 448.88±15.36 nm respectively by 15 minutes and 270.21±4.75 nm, 325.04±7.72 nm, 367.78±11.51 nm, and 416.85±16.28 nm respectively by 30 minutes of ultrasound (P<0.05).The PDI value of the samples without ultrasound was found 0.755±0.132 as compared to 15min (0.4720±0.50) and 30min 0.476±0.046. which shows that samples with ultrasound treatment had relatively narrow size distribution. Among different ratios the ultrasound treatment significantly (P<0.05) decreased the zeta potential of the native sample 1:4 (-28.54 ± 54 mV) to (-33.36 ± 0.85 mV) with 15 min and (-31.13±1.02 mV) with 30min ultrasound. This shows more colloidal stability with ultrasound treatment. Flow ramp data showed that the viscosity of the ultrasound treatment samples for 15 and 30min time was significantly (P< 0.05) decreased as compared to untreated samples. The apparent viscosity of the all samples exhibited shear thinning behavior in the range of (50-1000 s-1). All the flow behavior of samples was fitted with Sisko models (R2>0.997). The flow behavior index of the samples showed the pseudoplastic behavior (n< 1) and remained pseudoplastic after ultrasound treatment. The consistency index (Ks) of the samples was increased by ultrasound treatment.
Page(s):
108-108
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022