-The objective of the current study was to determine the activity of antioxidant enzymes: superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione peroxidase GPX and catalase (CAT) in 10 wheat near isogenic lines (NILs) showing compatible and incompatible interactions. The enzyme activity was determined at 24 hours after inoculation (24hai), 48 hours after inoculation (48hai) and 72 hours after inoculation (72hai). To fulfill this objective, 12-day-old wheat seedlings were inoculated with stripe rust race. The results of the study showed that enzyme activity and their gene expression was significantly high in incompatible interaction as compared to compatible interaction. All enzyme exhibited increased activity at 72hai. High enzyme activities of CAT (55.95 U/mg protein), APX (55.85 U/mg protein), SOD (97.64 U/mg protein) and GPX (84.75 U/mg protein) were recorded in Yr 5/6* Avocet S at 72hai. The results indicate a unique pattern of activity of the antioxidant enzymes in the compatible and incompatible interaction.