The Impact of pH and Growth Phases on Photosynthetic Pigments and Carotene of Coelastrella saipanensis N. Hanagata (Scenedsmacese, Shaerophleales) | [Journal of Global Innovations in Agricultural Sciences • 2024]

Author(s):

1. Zeina Gany Fadeel: Department of Biology, College of Education for Pure Sciences, University of Diyala,,Iraq

2. Fikrat M. Hassan: Department of Biology, College of Science for Women, University of Baghdad,Baghdad -,Iraq

3. M.M.I. Al-Mahdawe: Department of Biology, College of Education for Pure Sciences, University of Diyala,,Iraq

Abstract:

The present study aimed to investigate the effects of level pH and the growth phases of Coelastrella saipanensis on Chlorophyll a,b, total, and Carotene. The algae were cultured in BG11 media and grown at different pH levels. We measured chlorophyll a, b, total chlorophyll, growth phases, and carotene concentrations. The results showed that at pH 8.5, the measurements of photosynthetic pigments-chlorophyll a, Chlorophyll b, and the total chlorophyll (0.183, 0.268, and 0.433 mg L-1, respectively). The highest values of chlorophyll a (0.185 mg L-1), and b (0.339 mg L-1), and the total chlorophyll (0.492 mg L-1) were recorded in the stationary phase. In addition, the study found that at pH 8.5 and the beginning of the stationary phase, the highest levels of chlorophyll a, b, and total chlorophyll were 0.216, 0.398, and 0.603 mg L-1, respectively, in correlation with pH-cell harvesting phase interaction. Similarly, carotene concentrations were increased as photosynthetic pigments and recorded the highest values (2.533 mg L-1) at pH 8.5, and 3.275 mg L-1 at the stationary phase. Therefore, the algal C. saipanensis is a vital source of carotene to support nutrition and health It is used as an antioxidant.

Page(s): 693-700

DOI: 10.22194/JGIAS/24.1378

Published: Journal: Journal of Global Innovations in Agricultural Sciences, Volume: 12, Issue: 3, Year: 2024

Keywords:

chlorophyll , nutritional support , Carotene , Growth phases , pH , Coelastrella saipanensis

References:

[1] Abed I.J.,Abdulhasan G.A.,Najem A.M. .2018 .Genotype versus phenotype to determine the definitive identification of the genera Chlorella beijerinck, 1890 (chlorellaceae) and Coelastrella chodat, 1922 (scendesmaceae). Bulletin of the Iraq Natural History Museum (P-. , : 101-111.

[2] Aburai S.,Ohkubo H.,Miyashita K.,Abe K. .2013 .Composition of carotenoids and identification of aerial microalgae isolated from the surface of rocks in mountainous districts of Japan. Algal Research, 2 : 237-243.

[3] Agócs A.,Deli J. .2011 .Pigments in your food. Journal of food composition and analysis, 24 : 757-759.

[4] AL-Rawi A.,Hassan F.M.,Alwash B.M. .2020 .In Vitro Stiumlation of Ergosterol from Coelastrella Terrestris by Using Squalene and Studying Antioxidant Effect. Systematic Reviews in Pharmacy 11., : .

[5] Al-Rawi A.,Alwash B.M.,Al-Essa N.E.,Hassan F.M. .2018 .A New Record Of coelastrella terrestris (reisigl) hegewald and n. Hanagata, 2002 (sphaeropleales, scenedesmaceae) in Iraq. Bulletin of the Iraq Natural History Museum (P-. , : 153-161.

[6] AL-Janaby N.A.A.,Qasim A.F. .2019 .Effect of pH Ammonium to nirtate ratio in nutrient solution on growth yield and quality of Gerbera flowers (Gerbera jamesonii L.) under soilless system. Tikrit Journal for Agricultural Sciences, 15 : 58-68.

[7] Andersen R.A.,Kawachi M. .2005 .Algal culturing techniques. (Chapter 6 ): Traditional microalgae isolation techniques. , 83 : 100.

[8] Arnon D.I. .1949 .Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology 24:1., : .

[9] Zittelli Chini,G. Chini,Lauceri R.,Faraloni C.,Benavides A.M. Silva,Torzillo G. .2023 .Valuable pigments from microalgae: phycobiliproteins, primary carotenoids, and fucoxanthin. Photochemical & Photobiological Sciences 1-57., : .

[10] De Clerck O.,M.D. Guiry F.,Leliaert Y.,Samyn H.,Verbruggen H. .2013 .Algal taxonomy: a road to nowhere. Journal of Phycology, 49 : 215-225.

[11] Eijckelhoff C.,Dekker J.P. .1997 .A routine method to determine the chlorophyll a, pheophytin a and β-carotene contents of isolated photosystem II reaction center complexes. Photosynthesis Research, 52 : 69-73.

[12] Mekinić Generalić,I. Generalić,Šimat V.,Rathod N.B.,Hamed I.,Čagalj M. .2023 .Algal carotenoids: Chemistry, sources, and application. Foods, 12 : 2768.

[13] Gupta A.K.,Seth K.,Maheshwari K.,Baroliya P.K.,Meena M.,Kumar A.,Vinayak V. .2021 .Biosynthesis and extraction of high-value carotenoid from algae. Frontiers in Bioscience-Landmark, 26 : 171-190.

[14] Hadi S.M.,Ibrahim K.M.,Yousif S.I. .2019 .Differential Expression for Genes in Response to Drought and Salinity in Ruta graveolens Plantlets. Jordan Journal of Biological Sciences 12., : .

[15] Jawad A.L.M. .1982 .Interactions between cyanobacteria and other microorganisms. , : .

[16] Kaufnerová V.,Eliáš M. .2013 .The demise of the genus Scotiellopsis Vinatzer (Chlorophyta). Nova Hedwigia, 97 : 415-428.

[17] Liu B.H.,Lee Y.K. .2000 .Secondary carotenoids formation by the green alga Chlorococcum sp. Journal of applied phycology, 12 : 301-307.

[18] Lutzu G.A. and N.T.,Dunford G.A. and N.T. .2018 .Interactions of microalgae and other microorganisms for enhanced production of high-value compounds. Frontiers in Bioscience-Landmark, 23 : 1487-1504.

[19] Amin Mohd,Karim S.F.R.,Yusof Y.A.,Muhammad K. .2023 .Effects of Metal Concentration, pH, and Temperature on the Chlorophyll Derivative Content, Green Colour, and Antioxidant Activity of Amaranth (Amaranthus viridis) Purees. Applied Sciences, 13 : 1344.

[20] Nayana K.,Sudhakar M.P.,Arunkumar K. .2022 .Biorefinery potential of Coelastrella biomass for fuel and bioproducts-A review. Biomass Conversion and Biorefinery, 1 : .

[21] Rimet F.,Bouchez A. .2012 .Life-forms, cell-sizes and ecological guilds of diatoms in European rivers. Knowledge and management of Aquatic Ecosystems, 406 : 12.

[22] Sandhya M. .2013 .Thin layer chromatography and high pressure liquid chromatography profiling of plant extracts of Viola odorata Linn. International Journal of Pharma and Bio Sciences 4., : .

[23] Schneider I.A.H.,Freitas A.P.P.,Schwartzbold A. .2011 .Biosorption of heavy metals by algal communities in water streams affected by the acid mine drainage in the coal-mining region of Santa Catarina state. Brazil. Minerals Engineering, 24 : 1215-1218.

[24] Sneath P.H.A. ,Sokal R.R.,Taxonomy Numerical .1973 .. , : .

[25] Tamura K.,Stecher G.,Peterson D.,Filipski A.,Kumar S.,Biology Molecular .2013 .MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. , : 2725-2729.

[26] Toshkova-Yotova T.,Georgieva A.,Alexandrov S.,Ivanova A.,Pilarski P.,Toshkova R. .2022 .Antitumor and antimicrobial activity of fatty acids from green microalga Coelastrella sp. BGV. South African Journal of Botany, 151 : 394-402.

[27] Wang Q.,Song H.,Liu X.,Li B.,Hu Z.,Liu G. .2019 .Morphology and molecular phylogeny of coccoid green algae Coelastrella sensu lato (Scenedesmaceae, Sphaeropeales), including the description of three new species and two new varieties. Journal of phycology, 55 : 1290-1305.

[28] Wang Q.,Song H.,Liu X.,Zhu H.,Hu Z.,Liu G. .2018 .Deep genomic analysis of Coelastrella saipanensis (Scenedesmaceae, Chlorophyta): Comparative chloroplast genomics of Scenedesmaceae. European Journal of Phycology, 54 : 52-65.

[29] Zhou Y.,Xu J.,R. M. McKay H. J. MacIsaac,Xu R.,Pei Y.,Chang X. .2023 .Comparative metabolomic analysis of exudates of microcystin-producing and microcystinfree Microcystis aeruginosa strains. Frontiers in Microbiology, 13 : 1075621.

Citations

Downloads

Views