The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae | [Pakistan Journal of Zoology • 2024]

Author(s):

1. Milena Vlahović: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

2. Dragana Matić: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

3. Marija Mrdaković: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

4. Larisa Ilijin: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

5. Anja Grčić: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

6. Aleksandra Filipović: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

7. Jelica Lazarević: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

8. Vesna Perić-Mataruga: Department of Insect Physiology and Biochemistry, Institute for Biological Research, “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd 142, Belgrade, Serbia.

Abstract:

A B S T R A C T Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful efects of cadmium, we need to examine changes at all diferent levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic efects of two cadmium concentrations, 10 and 30 µg Cd/g dry food, as well as a threeday recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute efects and chronic treatment at 30 µg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 µg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term efect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium efects to gain a better insight into the state of the or ganism under stress conditions.

Page(s): 339-346

DOI: 10.17582/journal.pjz/20211019131007

Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 1, Year: 2024

Keywords:

correlations , Plasticity , Gypsy moth , Cadmium treatments , Midgut mass

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