The effect of Coelomic fluid Stichopus Hermanii on isolated perfused rat hearts and the involvement of prostaglandin in its mechanism of action. | [Pakistan Journal of Biological Sciences • 2005]

Author(s):

1. W. T. Tan: Department of Pharmacy, Faculty of Allied Health Sciences, University Kebangsaan Malaysia, 53100 Kuala Lumpur, Malaysia

2. Yew Su Fong: Department of Pharmacy, Faculty of Allied Health Sciences, University Kebangsaan Malaysia, 53100 Kuala Lumpur, Malaysia

3. B. H. Ridzwan: Department of Biomedical Science, Faculty of Science, International Islamic University Malaysia, Gombak, 53100 Kuala Lumpur, Malaysia

Abstract:

The objective of this study was to investigate the effect of the coelomic fluid (freeze-dried) on the contractility, heart rate and coronary perfusion pressure of the isolated perfused rat hearts by using the Langendorff system. The second objective of the study was to investigate the involvement of prostaglandin in the mechanism action of the fluid. Isoprenaline (ISO) and sodium nitroprusside (SNP) were used as positive controls. The dose-response curve for ISO was determined in which 1.0 nmol ISO produced maximum contractility and heart rate. Then the effect of the coelomic fluid was determined followed by its dose-response curve. The fluid showed vasorelaxation effect on coronary arteries but not on the heart rate and contractility. The dose 1.0 mg fluid was chosen in the mechanistic study since it produced maximum vasorelaxation and this effect was similar to 70% effect of SNP (1x10-6 mol). The mechanistic studies were divided into 3 parts. First, the time-matched control study. The result showed two identical doses (1.0 mg) of the fluid injected repeatedly into the heart gave similar effect. This study being used as a standard for further mechanism studies. The second and third parts, followed similar protocol as in the time-matched control. The first dose of the fluid was injected into the heart perfused with Kreb-Henseleit solution without adding Indomethacin (cyclooxygenase inhibitor). The second dose was injected into the heart perfused with the solution added Indomethacin (0.01 mM). The result showed Indomethacin reduced the vasorelaxation effect of the fluid by 52%. In conclusion, the studies indicated showed the coelomic fluid of S. hermanii caused vasorelaxation effect on coronary arteries and the mechanism involved prostaglandin.

Page(s): 78-84

DOI: DOI not available

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 8, Issue: 1, Year: 2005

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