A Study on molecular detection of Theileria lestoquardi by PCR amplification in apparently healthy small ruminants from five districts of southern Punjab. | [Pakistan Journal of Zoology • 2015]

Author(s):

1. Madiha Fatima: Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan

2. Sadaf Saeed: Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan

3. Rehan Sadiq Shaikh: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan

4. Muhammad Ali: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan

5. Furhan Iqbal: Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan

Abstract:

The present study was designed to detect the prevalence of Theileria lestoquardi in small ruminants of Southern Punjab and to determine the risk factors associated with the spread of theileriosis, if any. A total of 115 blood samples from small ruminants including goat (n=66) and sheep (n=49) were collected from five sampling sites (Bahawalnagar, Dera Ghazi Khan, Layyah, Multan and Muzaffargarh districts) in Southern Punjab, Pakistan from randomly selected herds. Data on the characteristics of the animals (species, gender, age, tick presence or absence and health status) and various herd characters (location, size, composition, dog presence or absence and tick association with the dog if present) was collected on sampling sites through questionnaire. Prevalence of the T. lestoquardi was recorded to be 3.47% (n=4) through PCR amplification of their 18S rRNA gene that produced a 730 base pairs DNA fragment. All positive samples were sheep blood samples collected from Multan indicating that sheep were more prone to this parasite (P = 0.03). It was observed that tick association with the animal was a highly significant (P = 0.00003) risk factor associated with the theileriosis in small ruminants.

Page(s): 441-446

DOI: DOI not available

Published: Journal: Pakistan Journal of Zoology, Volume: 47, Issue: 2, Year: 2015

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views