Abstract:
Nickel oxide nanoparticles (NiO NPs) have attracted increasing attention owing to their potential use in a variety of applications as they are used as catalyst, sensor magnetic materials, printing inks, diesel fuel additive, battery cathodes, gas sensors, electrochromic films and dye sensitized photocathodes. Despite of their large scale use, it is expected that they might have lot more drawbacks as nanomaterials are known to cross the cell membranes more easily and effectively than their bulk forms owing to their very small sizes. Present study was aimed to document the effect of two different doses of NiO NPs on selected aspects of behavior, complete blood count, serum parameters and biomarkers of oxidative stress from vital organs of albino mice in a sex specific manner. NiO NPs were synthesized by hydrothermal method and crystalline nature, purity and surface morphology of synthesized material was confirmed by X-ray diffraction analysis and field emission electron microscopy respectively. The average particle size of synthesized NiO NPs was 43 nm. Five weeks old albino mice (N = 48) of both sex were intraperitoneally injected either with low dose (20 mg) or higher dose (50mg/ ml saline/ Kg body weight) of NiO NPs for 14 days. Control groups were maintained in parallel and they were intraperitoneally treated with saline solution. A series of behavioral tests (Rota Rod test, Open Field test, Light and Dark Box test and Novel Object test) were conducted in all subjects. After behavioral studies, mice were sacrificed under Isoflurane anesthesia and blood samples were collected for complete blood count and serum analysis. Vital organs (liver, heart, kidney, brain and lungs) were also removed surgically for the subsequent analysis of Nickel concentration and markers of oxidative stress in the tissue homogenates. We are reporting that the intraperitoneal injections of NiO NPs for 14 days can affect the muscular activity, object recognition capacity and exploratory behavior of albino mice in a sex specific manner. The behavioral effects were more pronounced at higher dose and in female mice. NiO NPs drastically affect blood serum parameters and oxidative stress biomarkers from vital organs of albino mice. Toxic effects of NiO NPs were again dose and sex dependent and they were more pronounced at higher dose and in male mice. We recommended that the biocompatibility of NiO NPs should be further explored at the molecular level under variable experimental conditions so that their biologically save levels must be determined as it is involve in the manufacture of several materials that are in our daily use.
Page(s):
DOI:
DOI not available
Published:
Journal: Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 , Volume: 0, Issue: 0, Year: 2023