Abstract:
Elevated levels of reactive oxygen species (ROS) pose severe threats to intracellular environment in terms of oxidative stress and their continuous overproduction causes various clinical disorders. Antioxidant compounds combat oxidative stress in cells and are therefore useful in management of various clinical disorders. Over the past few years, several polysaccharides have been extracted from plants and studied for various biomedical applications. Lepidium sativum, commonly known as garden cress, is a mucilaginous plant whose seeds release mucilage upon soaking in water. The present study aimed to extract and partially purify water soluble polysaccharides from seed coat mucilage of garden cress via size-exclusion chromatography and to evaluate the bioactive fraction. The mucilage was extracted by soaking cress seeds in double distilled water (ddH2O). The mucilage was dried and precipitated in 75% ethanol. The ethanol precipitated fraction was then re-dissolved in ddH2Oand fractionated through Bio-Gel P-10 (90 µm-180 µm). A total of 60 fractions were obtained and processed for quantification of total carbohydrates contents. The results revealed the presence of pentoses, hexoses, saturated, and unsaturated uronic acid contents. Antioxidant potential of the cress polysaccharides was performed by DPPH free radical scavenging, hydrogen peroxide scavenging and Invitro lipid peroxidation assays. The fractionF23 exhibited strong antioxidant potential by scavenging the DPPH free radical with an effective EC50value of 37.93 µg/mL. Similarly, fraction F53 at 200 µg/mL effectively scavenged the H2O2 free radical by 81.89%. Furthermore, invitro lipid peroxidation assay was performed on mice tissue samples, where fraction F52 was the most effective one having IC50 value of 34.17 µg/mL. The results conclude that the cress polysaccharides have uronic acid as a major monosaccharide unit, which might be responsible for its significant antioxidant potential. Moreover, the polysaccharides from cress seed coat mucilage can be used as potential and effective drug against oxidative stresses.
Page(s):
88-88
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
antioxidant
,
mucilage
,
Reactive oxygen species
,
Polysaccharides
,
Lepidium sativum