Genotoxicity and carcinogenicity of Frosskaolea tenacissima (Linn) leave extract used in traditional medicine in Saudi Arabia | [Bioscience Research • 2023]

Author(s):

1. Sameer H. Qari: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

2. Ahmed B. Ashour: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

3. Kamal A. Attia: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

4. Abdulmajeed F .Alrefaei: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

5. Wessam M. Filfilan: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

6. Mohamed E. Elbeeh: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

7. Alaa T. Qumsani: Biology Department, Aljumum University College, Umm Al-Qura University,Makkah,Saudi Arabia

Abstract:

The present work aimed to evaluate the potential carcinogenic, cytotoxic, and genotoxic effects of methanolic extract leaves of Frosskaolea tenacissima L. found in Makkah region, Saudi Arabia. The Mitotic Index (MI) was determined for cytotoxicity studies, and micronuclei (MN), chromosomal aberrations (CA) were counted, and a molecular marker (ISSR-PCR assay) was performed to study the genotoxicity, and then the DNA amount and fragments in comet cells were figured for the carcinogenicity of each 0.27, 0.55, 1.10, and 1.37 mg/ml concentration of the crude extracts of F. tenacissima using the Allium cepa In Vivo bioassay. The results showed that F. tenacissima methanolic extract exerted significant cytotoxic and genotoxic effects on A. cepa cells with significant values for concentrations from 0.55 mg/ml, 1.10 mg/ml, and 1.37 mg/ml. It was found that the low growth of the meristematic cells of A. cepa roots, and increased significantly the mitotic index compared with the control, and also, the high micronucleus and chromosomal aberrations count showed the presence of genotoxicity/ or cytotoxicity, as well as a low number of DNA damage in comet cells, which indicated dose-dependent carcinogenic potential effects of this plant. These results suggest F. tenacissima is expected to have the same effects on human cells. So, these mutagenic and genotoxic effects on human cells should be investigated in further studies.

Page(s): 789-799

DOI: DOI not available

Published: Journal: Bioscience Research, Volume: 20, Issue: 3, Year: 2023

Keywords:

Allium cepa , carcinogenicity , genotoxicity , cytotoxicity , Frosskaolea tenacissima

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