X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression | Journal of Pakistan Medical Association 2023

Author(s)

Jin-xiao Liang Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

Wei Gao School of Medicine, Zhejiang University City College, China.

Wei-tian Wei Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

Xun Yang Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

Jin-shi Liu Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

Abstract

Objective: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation. Method: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5. Results: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y. Conclusion: There was a significant role of glycosylation in radiation therapy for lung cancer.

Publication Details

Page(s) 494-499

DOI 10.3322/caac.21590

Published Journal: Journal of Pakistan Medical Association, Volume: 73, Issue: 3, Year: 2023

Keywords

XRay Lung cancer SP1 Fucosyltransferase 4 Cell proliferation

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