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Enhancing extraction efficiency and biological activities of Syzygium aromaticum essential oils: a comparative study of conventional extraction techniques with superheated steam distillation
Author(s):
1. M. Adnan Ayub: Department of Chemistry, University of Sahiwal, Sahiwal 57000, Pakistan
2. Haseeb Raza: Department of Chemistry, University of Sahiwal, Sahiwal 57000, Pakistan
3. Ambreen Fatima: Department of Chemistry, University of Sahiwal, Sahiwal 57000, Pakistan
4. M. Shahid: Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan
5. M. Amin Abid: Department of Chemistry, University of Sahiwal, Sahiwal 57000, Pakistan
Abstract:
Syzygium aromaticum L. is an aromatic plant with a significant amount of essential oil (EO), which is used in food, medicine, for flavoring, and in the fragrance industry. The purpose of this study was to comparatively evaluate the chemical composition, yield, and antioxidant and antifungal activities of Syzygium aromaticum essential oils extracted by the conventional hydrodistillation, steam distillation, and the emerging superheated steam distillation methods. It was noticed that the extraction methods significantly influenced the yield, chemical composition, and antioxidant and antimicrobial activities of essential oils. The maximum yield was obtained using superheated steam distillation, followed by hydro-distillation and steam distillation. The antioxidant potential of EO extracts was evaluated following the scavenging of 2,2-dipenyl-1-picrylhydrazyl radicals, hydrogen peroxide scavenging activity and ferric reducing power assays. Results revealed that EO extracted superheated steam distillation exhibited the highest antioxidant activity. GC-MS analysis depicted eugenol (47.94-26.50%) and caryophyllene (20.24-9.25%) as the major compounds of Syzygium aromaticum EOs. The antimicrobial activity of EO extracts was evaluated, via the resazurin microtiter plate assay, microdilution broth assay, and disc diffusion methods, against normal and food pathogenic bacterial and fungal strains. After comparative evaluation, it was observed that superheated steam extracted EO exhibited the highest antimicrobial potential. Overall, methodical evaluation disclosed that superheated steam distillation is an effective method to extract EOs from plant sources, with greater yield and promising biological activities.
Page(s): 41-41
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
essential oil , Syzygium aromaticum , superheated steam distillation , caryophyllene
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