Abstract:
TANK binding kinase 1-binding protein 1 is protein which has numbers of protein-binding sites, to which TBK1 and IKBKE bind and perform different functions mainly immuno-modulatory functions, anti-proliferative and antiviral innate-immunity in which the interferons Type-1(IFN) such as IFN-ß and IFN-a are released, due to which this protein is called adapter protein. It is observed to localize in lungs, small intestine, leukocytes, heart, placenta, muscles, kidneys and relatively low level in thymus and brain. It is a protein which is encoded by gene TBK1 present in Homo sapiens. Present study was conducted to predict3D structure of protein through different online servers that would be helpful for further studies in future. The 3D structure of protein predicted through online Software tools such as I-TASSER, Robetta and Alpha-Fold. These tools provide approximately 5structures each of which was then evaluated and validated by another tool SAVES (VERSION6.0). The resulting structures from different tools were validated and evaluated by SAVES (VERSION 6.0). After validation the best predicted structure found through Robetta that had ERRAT value of 84.0604 and PROCHECK (Ramachandran plot) had found residues in most favorable regions were 90.8 %, residues in additional allowed regions were 8.0%, residues in generously allowed regions were 0.6% and residues in disallowed regions were 0.6%. The predicted model was then visualized through Swiss PDB Viewer in different format such as ribbon and ball and stick. After unveiling 3D structure of novel protein, we concluded that this structure will help us to find out its role other than in antiviral innate immunity and by producing torsion in its 3Dstructure, researchers will be able to detect either this protein is involved in any disease or not because according to previous studies it is not associated with any disease.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
3D structure prediction
,
InnateImmunity
,
TANK binding kinase 1
,
ITASSER server