Abstract:
Lipoxygenases (LOX) are the non-Sulphur, non-heme (iron) cofactor containing dioxygenasesthat play important roles in various biological processes including;inflammation, lipid metabolism and cell signaling. It alsoregulates the production of hormones and defensive metabolites in plants, animals and microorganisms (algae and fungi) due to their ability of catalyzing the oxidation of polyunsaturated fatty acids (PUFAs) such as linolenic, linoleic, and arachidonic acid. In chickens, lipooxygenase enzymes are involved in the synthesis of bioactive compounds called eicosanoids from PUFAs. Recent work focused on the isolation and partial purification of LOX from the heart, liver, and brain tissues of Gallus gallus domestics (chicken), as well as the determination of its biochemical parameters. The LOX enzyme was purified from the heart, liver, and brain tissues of Gallus gallus domestics usingphosphate(PO4) buffer at pH=9, and it was subsequently concentrated using the salt precipitation method with ammonium sulphate to levels of 30% and 70%. The specific activity of LOX in the heart, liver, and brain tissues was (2.522, 5.539, and 3.03) U/mg, (9.852, 6.602, and 7.096) folds purification, with (79.22, 56.33, and 49.80)% recovery, respectively. The optimum temperature for enzyme was 40 oC for all three samples whereas the optimum pH for each organ varied from 5.2 for the heart to 4.8 for the liver and brain. The preliminary results obtained from the biochemical characterization of LOX from three organ tissues (heart, liver and brain) of Gallus gallus domestics can serve as a starting point for future in-depth studies.
Page(s):
64-64
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
enzymatic activity
,
Gallus gallus domestics chicken
,
PUFAs
,
Polyunsaturated fatty acids
,
salt precipitation
,
Lipoxygenases LOX