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A biosynthesis method to produce resveratrol in saccharomyces cerevisiae with secretion expression of grape anti-oxidant resveratrol synthase gene during wine fermentation.
Author(s)
YANGBO SONG College of Enology, Northwest A&F University,Yangling, Shaanxi, 712100,P. R. China
LI FENG College of Enology, Northwest A&F University,Yangling, Shaanxi, 712100,P. R. China
DONGQING YE College of Enology, Northwest A&F University,Yangling, Shaanxi, 712100,P. R. China
YUYANG SONG College of Enology, Northwest A&F University,Yangling, Shaanxi, P. R. China; Shaanxi Engineering Research Center for Viti-Viniculture,Yangling, Shaanxi, P. R. China
YI QIN College of Enology, Northwest A&F University,Yangling, Shaanxi, 712100,P. R. ChinaShaanxi Engineering Research Center for Viti-Viniculture,Yangling, Shaanxi, 712100,P. R. China
YANLIN LIU College of Enology, Northwest A&F University,Yangling, Shaanxi, 712100,P. R. ChinaShaanxi Engineering Research Center for Viti-Viniculture,Yangling, Shaanxi, 712100,P. R. China
SHUWEN LIU College of Enology, Northwest A&F University,Yangling, Shaanxi, P. R. China; Shaanxi Engineering Research Center for Viti-Viniculture,Yangling, Shaanxi, P. R. China
Abstract
Resveratrol (Res), a stilbenic compound, recently has become the focus of a number of studies in medicine and plant physiology, and Saccharomyces cerevisiae (S. cerevisiae) has become a popular host for production of non-native compounds. As the last and key enzyme of the biosynthesis pathway, the amount and expression level of Resveratrol Synthase (RS) positive correlation on the production of Res. In this study, a signal peptide sequence, secreted by a-factor of S. cerevisiae, was connected with the RS gene, resulting a pYCa secretory plasmid construction. The RS gene was secretory expressed in S. cerevisiae successfully. Subsequently, a white wine fermentation was carried out by the recombinant strain. The Res concentration of the white wine fermented by the recombinant strain was 36% higher than the control. This has great interests for establishing a controllable way to produce RS and lays the foundation for exploring the Res biosynthesis method.
Publication Details
Page(s) 2443-2448
DOI DOI not available
Published Journal: Pakistan Journal of Botany, Volume: 50, Issue: 6, Year: 2018
Keywords
Saccharomyces cerevisiae Resveratrol synthase Secretion expression
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