Microsatellite Based Genetic Diversity among Different Populations of Taxus Baccata from Neelum Azad Kashmir, Pakistan | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Wasim Akhtar: Department of Botany University of Azad Jammu and Kashmir Muzaffarabad

2. Komal Qammar: Department of Botany University of Azad Jammu and Kashmir Muzaffarabad

3. Iram Fatima: Department of Biotechnology FJWU Rawalpindi, Pakistan

4. Ejaz Aziz: Department of Botany, Khanpur Degree College KP, Pakistan

5. Imran Hamza: Department of Botany University of Kotli AJK, Pakistan

6. Amir Shahbaz: Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan

7. Ammara Munir: Department of Biotechnology University of Azad Jammu and Kashmir Muzaffarabad, Pakistan

Abstract:

Taxus baccata L. is an evergreen tree of order Coniferales. It is small to medium sized tree which has value wood and forage for cattle. It is also native to Himalaya's regions and mixed with fir and pines. In Azad Jammu and Kashmir (AJK), T. baccata has low diversity due to deforestation, over exploitation and other human's impacts. Owing to the endangered status of T. baccata, a study has been designed to access its genetic diversity based on simple sequence repeats (SSR) markers. SSR were designed and amplified using Polymerase chain reaction (PCR). The data was scored from amplified bands and it was shown that T. baccata from district Neelum AJK has no polymorphism. Most of the bands were mono-morphic and only two were dimorphic. This low level of genetic diversity can be attributed to human impact on T. baccata populations. From the results and conclusion, it is suggested that T. baccata in AJK needs restoration as early as possible. A comprehensive study is needed to estimate its genetic diversity from whole country and restoration approaches.

Page(s): 43-43

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

Microsatellite loci , Microsatellite loci , Endangered , Genetic variations , Taxus

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