VTE1 and VTE3 gene expression during vitamin e production in sunflower (Helianthus annuus L.) treated with different fertilization fertilization | [Pakistan Journal of Biological Sciences • 2023]

Author(s):

1. Habib Hasan Amimy: Universitas Andalas, Padang 25163, West Sumatera, Indonesia

2. Roza Yunita: Department of Agronomy, Faculty of Agricultural, Universitas Andalas, Padang , West Sumatera, Indonesi

3. Rauza Sukma Rita: Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang 25163, West Sumatera, Indones

4. Firsta Ninda Rosadi: Department of Agronomy, Faculty of Agricultural,Universitas Andalas, Padang , West Sumatera,Indonesia

5. Lily Syukriani,: Department of Agronomy, Faculty of Agricultural,Universitas Andalas, Padang 25163, West Sumatera,Indonesia

6. Suhada Tri Marneli,: Department of Agronomy, Faculty of Agricultural, Universitas Andalas, Padang , West Sumatera, Indonesia

7. Amalia Azizah: Department of Agronomy, Faculty of Agricultural, Universitas Andalas, Padang , West Sumatera, Indonesia

8. Jamsari: Universitas Andalas, Padang 25163, West Sumatera, Indonesia;Department of Agronomy, Faculty of Agricultural, Universitas Andalas, Padang 25163, West Sumatera, Indonesia

Abstract:

Background and Objective: Sunflower is one of the important commodities in agriculture. The oil contentin sunflower seeds has been widely used as cooking oil, butin Indonesia, the utilization of this oil is still relatively low. In addition, sunflowers also contain vitamin E which is useful as an antioxidant, so it can be used to reduce the risk of cardiovascular disease. This study aims to determine gene expression at the RNA level towards vitamin E biosynthesis using different fertilization treatments. Materials and Methods: Sunflowers that had been given different fertilizers were taken in three flowering phases, R3, RS and R8. Flower samples were isolated until RNA was obtained. The isolation results were tested using real-time PCR to determitnhee relative gene expression of the V/7£7and V7E3genes. After the sunflower seweerefdullyrsipe, vitamin E con twasetnestted in each treatment and the results were compared with the relative gene expression obtained. Results: The results obtained were fluctuating, but in general, the relative gene expression obtained in the VTETgeneincreasedin the R3 phase and then decreasedin the RS and R8 phases. Whereas, in the I/7E3genteh,e relative gene expression obtained experienced an increase in the R3 and R5 phases and then decreased in the R8 phase. The highest vitamin E content was obtained by sample P3 (4218 ug mL"") and the lowest was obtained by sample P2 (1798 pg mL""). Conclusion: A balanced ratio of 92:46:30 kg ha~" of major nutrient fertilizer involving N, P and K could increase vitamin E content in sunflowers. Such a combination exhibited stable expression of the [/7£7and I/7E3genes in all phases of flowering.

Page(s): 409-418

DOI: 10.3923/pjbs.2023.409.418

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 26, Issue: 8, Year: 2023

Keywords:

fertilization , Sunflower , V77 , V7E3 gene expression

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