A C2H2 zinc finger protein GhZFP8 from cotton (Gossypium hirsutum) is involved in salt stress tolerance in arabidopsis | [Pakistan Journal of Botany • 2024]

Author(s):

1. ZHI-HUI YUAN: College of Chemistry and Bioengineering, Hunan University of Science and Engineering,Yongzhou 425199, Hunan,China

2. YING LI: College of Bioengineering, Jingchu University of Technology,Jingmen 448000, Hubei,China

3. BIN ZHANG: College of Chemistry and Bioengineering, Hunan University of Science and Engineering,Yongzhou 425199, Hunan,China

4. WEN-HAN CHENG: College of Bioengineering, Jingchu University of Technology,Jingmen 448000, Hubei,China

5. YONG-CHANG LIU: College of Bioengineering, Jingchu University of Technology,Jingmen , Hubei,China

Abstract:

C2H2-type zinc finger proteins (ZFP) function in many biological processes. In this study, GhZFP8 encoding a C2H2 ZFP was cloned from cotton. Results of qPCR indicated that the transcripts of GhZFP8 were detected in the leaves and fibers of 3, 6, and 30 days post anthesis, but not in the roots, stems, and flowers. The transcriptional level of GhZFP8 was regulated by cold, salt, PEG, and ABA. Sub-cellular localization analysis showed that GhZFP8 was distributed in the nucleus of tobacco leaves. GhZFP8 transgenic Arabidopsis exhibited improved tolerance to high salinity but less sensitivity to ABA. Meanwhile, overexpression of GhZFP8 elevated the rate of water loss in leaves compared to that in wild types. All results suggested that GhZFP8 may function in the response of Arabidopsis to salt and ABA in cotton.

Page(s): 35-41

DOI: 10.30848/PJB2024-1(8)

Published: Journal: Pakistan Journal of Botany, Volume: 56, Issue: 1, Year: 2024

Keywords:

Salt stress , Gossypium hirsutum , Zinc finger protein , C2H2type , ABA

References:

[1] Chang T.,Yu Q.,Yang C.,Li C.,Xiong S.,Gao Q.,Xie F.,Zheng H.,Li Z.,Tian C.,Yang Z.,Ye Z. .2018 .Hair, encoding a single C2H2 zinc-finger protein, regulates multicellular trichome formation in tomato. Plant, 96(1) : 90-102.

[2] Ciftci-Yilmaz S.,Mittler R. .2008 .The zinc finger network of plants. Cell Mol. Life Sci, 65(7-8) : 1150-1160.

[3] Deng H.,Liu H.,Li X.,Xiao J.,Wang S. .2012 .A CCCHtype zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease. Plant Physiol, 158(2) : 876-889.

[4] Gan Y.,Kumimoto R.,Liu C.,Ratcliffe O.,Yu H.,Broun P. .2006 .GLABROUS INFLORESCENCE STEMS modulates the regulation by gibberellins of epidermal differentiation and shoot maturation in Arabidopsis. Plant, 18(6) : 1383-1395.

[5] Gan Y.,Liu C.,Yu H.,Broun P. .2007 .Integration of cytokinin and gibberellin signalling by Arabidopsis transcription factors GIS, ZFP8 and GIS2 in the regulation of epidermal cell fate. Development, 134(11) : 2073-2081.

[6] Guo Y.H.,Yu Y.P.,Wang D.,Wu C.A.,Yang G. D.,Huang J.G.,Zheng C.C. .2009 .GhZFP1, a novel CCCHtype zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5. , 183(1) : 62-75.

[7] Han Y.,Zhang A.,Huang L.,Yu X.,Yang K.,Fan S.,Cao J. .2011 .BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp. Chinensis. Mol. Biol. Rep, 38(8) : 5321-5325.

[8] Han Y,Zhou H.Y.,Xu L.A.,Liu X.Y.,Fan S.X.,Cao J.S. .2018 .The zinc-finger transcription factor BcMF20 and its orthologs in Cruciferae which are required for pollen development. Biochem. Biophys. Res. Comm., 503 : 998-1003.

[9] Song B.,Yu Y. Zhang.,Fu Y.P.,Wang P.W. .2019 .Novel drought-inducible Cys2/His2-type zinc finger protein STF-2 from soybean (Glycine max) enhances drought tolerance in transgenic plants. Pak. J. Bot., 51(3) : 823-829.

[10] Kodaira K.S.,Qin F.,Tran L.S.,Maruyama K.,Kidokoro S.,Fujita Y.,Shinozaki K.,Yamaguchi-Shinozaki K. .2011 .Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions. Plant, 157(2) : 742-756.

[11] Kubo K.,Sakamoto A.,Kobayashi Z.,Rybka Y.,Kanno H.,Nakagawa H.,Takatsuji H. .1998 .Cys2/His2 zinc-finger protein family of petunia: evolution and general mechanism of target-sequence recognition. Nucl. Acids Res, 26(2) : 608-615.

[12] Li G.,Tai F.J.,Zheng Y.,Luo J.,Gong S.Y.,Zhang Z.T.,Li X.B. .2010 .Two cotton Cys2/His2-type zinc-finger proteins, GhDi19-1 and GhDi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling. Plant Mol. Biol, 74(4-5) : 437-452.

[13] Najafabadi S.,Mnaimneh F.W.,Schmitges M.,Garton K.N.,Lam A.,Yang M.,Albu M.T.,Weirauch P.M.,Kim J.,Greenblatt B.J.,Frey T.R.,Hughes T.R. .2015 .C2H2 zinc finger proteins greatly expand the human regulatory lexicon. Nat. Biotechnol, 33(5) : 555-562.

[14] Prigge M.J.,Wagner D.R. .2001 .The Arabidopsis SERRATE gene encodes a zinc-finger protein required for normal shoot development. Plant Cell, 13(6) : 1263-1279.

[15] Ren G.,Li L.,Huang Y.,Wang Y.,Zhang W.,Zheng R.,Zhong C.,Wang X. .2018 .GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin. , 219(2) : 728-742.

[16] Shi X.,Gu Y.,Dai T.,Wu Y.,Wu P.,Xu Y.,Chen F. .2018 .Regulation of trichome development in tobacco by JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas L. Gene. , 658 : 47-53.

[17] Sun S.Q.,Guo X.,Yang Y.M.,Bao H.J.,Tang H.,Sun J.,Huang H.S.,Zhang H.S. .2010 .Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice. J. Exp. Bot., 61(10) : 2807-2818.

[18] Wang D.,Yang J. .2002 .Cloning and characterization of cDNA encoding cotton STZ-like protein. , 41 : 42-46.

[19] Xu S.,Wang X.,Chen J. .2007 .Zinc finger protein 1 (ThZF1) from salt cress (Thellungiella halophila) is a Cys2/His-2-type transcription factor involved in drought and salt stress. Plant Cell Rep, 26(4) : 497-506.

[20] Yamaji C.F.,Huang S.,Nagao M.,Yano Y.,Sato Y.,Nagamura J.F.,Ma J.F. .2009 .A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. Plant Cell, 21(10) : 3339-3349.

[21] Yuan X.,Huang P.,Wang R.,Li H.,Lv X.,Duan M.,Tang H.,Zhang H.,Huang J. .2018 .A zinc finger transcriptional repressor confers pleiotropic effects on rice growth and drought tolerance by down-regulating stress-responsive genes. Plant Cell Physiol, 59(10) : 2129-2142.

[22] Zhang S.,Lin T.,Zhang L.,Pei Q.,Tang F.,Liu Y.,Liu Y. .2015 .Cloning and Expression Analysis of GhSIZ1, Encoding a C2H2 Zinc Finger Protein in Cotton. , 27(3) : 189-197.

[23] Zhang Z.,Sun C.,Ma Q.,Bu H.,Chong K.,Xu Y. .2018 .A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice. J. Plant Physiol, 229 : 100-110.

[24] Zhou Z.,An L.,Sun L.,Zhu S.,Xi W.,Broun P.,Yu H.,Gan Y. .2011 .Zinc finger protein 5 is required for the control of trichome initiation by acting upstream of zinc finger protein 8 in Arabidopsis. Plant, 157(2) : 673-682.

[25] Zhou Z.,Sun L.,Zhao Y.,An L.,Yan A.,Meng X.,Gan Y. .2013 .Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana. , 198(3) : 699-708.

Citations

Downloads

Views