The Use of Genetic Merit and Allelic Frequencies to Alter bTB Susceptibility and Disease Progression in Cow Herds | [Pakistan Journal of Zoology • 2025]

Author(s):

1. Bejaoui Safa: National Agronomic Institute of Tunis, University of Carthage, Tunisia University of Carthage, LR13AGR02, Mateur Higher School of Agriculture, Mateur 7030, Tunisia;Institution of Agricultural Research and Higher Education (IRESA), Mateur Higher School of Agriculture, Mateur 7030, Tunisia

2. Ferchichi Mohamed Amine: University of Carthage, LR13AGR02, Mateur Higher School of Agriculture, Mateur 7030, Tunisia;Institution of Agricultural Research and Higher Education (IRESA), Mateur Higher School of Agriculture, Mateur 7030, Tunisia

3. Jemmali Bayrem: University of Carthage, LR13AGR02, Mateur Higher School of Agriculture, Mateur 7030, Tunisia;Institution of Agricultural Research and Higher Education (IRESA), Mateur Higher School of Agriculture, Mateur 7030, Tunisia

Abstract:

The study aimed to characterize the relationship between Holstein cows’ TLR2 gene polymorphisms and their susceptibility to tuberculosis. In total, 200 Tunisian Holstein cows were used, in wich 100 control and 100 cases. PCR-RFLP and direct sequencing PCR product were used to characterize genetic polymorphisms of TRL2. Univariate logistic regression analysis was performed to characterize the relationship between studied variables and the bTB status for analyzed animals. PROC LOGISTIC method was utilized to ascertain the genotypic and allelic frequencies that were associated with bTB status. The odds ratio (OR) of wild genotypes compared to muted genotypes was computed. Results showed that single intradermal tuberculin test for bTB was not affected by the farm or the age factors (p>0.05). The allele frequency for studied animal was 72 % and 28 % respectively for G and T alleles. Statistical analysis confirms the significant (p 0.05) effect of the genotype at individual and herd level. The animal’s vulnerability against bTB was most likely associated with the G allele and bTB can be observed in GG genotype 10 % more than TT genotype. The identification of specific SNP can be a sure way to improve the genetic merit of cow herds against bovine tuberculosis incidence. The use of genetic merit and allelic frequencies can alter bTB susceptibility and disease progression in cow herds.

Page(s): 1573-1579

DOI: 10.17582/journal.pjz/20240205022049

Published: Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 4, Year: 2025

Keywords:

Tuberculosis , Polymorphism , Bovine , TLR2

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