Use of pefloxacin as a surrogate marker to detect ciprofloxacin susceptibility in Salmonella enterica serotypes Typhi and Paratyphi A | [Journal of Pakistan Medical Association • 2020]

Author(s):

1. Safia Moin: Department of Pathology and Laboratory Medicine, Aga Khan University Hospital,Karachi,Pakistan

2. Mohammad Zeeshan: Department of Pathology and Laboratory Medicine, Aga Khan University Hospital,Karachi,Pakistan

3. Sidra Laiq: Department of Pathology and Laboratory Medicine, Aga Khan University Hospital,Karachi,Pakistan

4. Ahmed Raheem: Department of Pathology and Laboratory Medicine, Aga Khan University Hospital,Karachi,Pakistan

5. Afia Zafar: Department of Pathology and Laboratory Medicine, Aga Khan University Hospital,Karachi,Pakistan

Abstract:

Objective: To determine the use of pefloxacin as a surrogate marker to detect fluoroquinolone (ciprofloxacin) susceptibility against Salmonella enterica serotypes Typhi and Paratyphi A. Methods: The prospective, descriptive cross-sectional study was conducted at the Aga Khan University Hospital, Karachi, from September 2016 to March 2018, and comprised Salmonella Typhi and Paratyphi A isolates of blood cultures. Disk susceptibility tests and broth microdilution to test minimum inhibitory concentration were performed as per standard guidelines. Data was analysed using SPSS 21. Results: Of the 138 isolates, 91(66%) were intermediate resistant to ciprofloxacin but were resistant to pefloxacin, 42(30%) were resistant to both ciprofloxacin and pefloxacin, and 5(4%) were susceptible to both ciprofloxacin and pefloxacin. Of the isolates that were intermediate resistant to ciprofloxacin, 85(93%) had minimum inhibitory concentration range0.12-0.5mg\L, while 6(7%) had MIC >1mg\L (p<0.0001). Conclusion: Pefloxacin disk diffusion test was found to be reliable in detecting fluoroquinolone resistance among enteric fever causing Salmonella.

Page(s): 96-99

DOI: DOI not available

Published: Journal: Journal of Pakistan Medical Association, Volume: 70, Issue: 1, Year: 2020

Keywords:

ciprofloxacin , Fluoroquinolones , Pefloxacin , Enteric fever causing Salmonella

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