Isolation of Monochloroacetic Acid Biodegrading Bacteria from Tigris River | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2022]

Author(s):

1. Sahira N. Muslim: Department of Biology, College of Science, Mustansiryiah University, Baghdad, Iraq

2. Wafaa Hassan Muslem: Department of Biology, College of Science, Mustansiryiah University, Baghdad, Iraq

3. Thana Majid Zayer Noor: Department of Biology, College of Science, Mustansiryiah University, Baghdad, Iraq

4. Hassan Muslem Abdulhussein: Department of Radiology, Dijlah University College, Baghdad, Iraq

5. Mohamed Faraj Edbeib: Department of Animal Production, Faculty of Agriculture, Baniwalid University, Libya

6. Roswanira Abdul Wahab: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Malaysia

7. Fahrul Huyop: Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Malaysia

Abstract:

Organic compounds containing halogens are widely dispersed throughout the world, resulting in diferent types of pollution. One of the most common xenobiotics used in agricultural activities is monochloroacetic (MCA) which was Isolated from Iraqi mud in the Tigris River. This bacterial strain was known as SW2. Both standard universal primers Fd1 and rP1 were used with the colony PCR method for bacterial identification before being sent out for sequencing. Basic Local Alignment Search Tool nucleotide sequences and information were analyzed (BLASTn). The phylogenetic tree was constructed using the 16S rRNA sequence to determine their evolutionary distance. The Neighbor-Joining method was used to infer the evolutionary history. The Neighbor-Joining method was used to infer the evolutionary history. There is a 96 percent match between the SW2 bacterium and another type of aerobic GramNegative Bacteria. Strain SW2 (Pseudomonas sp.) was inoculated for two days and yielded colonies that were small, non-spore-forming, and rod-shaped. Growth slowed slightly after 48 hours. The release of chloride ions as a result of the degradation of MCA was seen using a halide ion test. Biochemical tests backed up the choice of the genus's name as well. As a result, bacteria found in the river are capable of utilizing and degrading the MCA compound. In conclusion, this study confirmed the potential ability of the isolated bacterial strain of utilizing MCA, especially from contaminated environments with the pragmatic application of the bacterial strains to degrade residual herbicide.

Page(s): 53-59

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 59, Issue: 4, Year: 2022

Keywords:

Degradation xenobiotic , Pseudomonas sp SW2 , Monochloroacetic MCA , Dehalogenase

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