The effect of melatonin on the efficiency of regeneration and gene expression during the morphogenesis in rice | [International Journal of Agriculture and Biology • 2023]

Author(s):

1. Nanang Tri Haryadi: University of Jember,Jember, East Java,Indonesia

2. Nanda Adya Sasmita: University of Jember,Jember, East Java,Indonesia

3. Mitha Aprilia Mufadilah: University of Jember,Jember, East Java,Indonesia

4. Nurhaliza Thamrin: University of Jember,Jember, East Java,Indonesia

5. Nabila Nur Aisyah Al Ayyubi: University of Jember,Jember, East Java,Indonesia

6. Nilasari Dewi: University of Jember,Jember, East Java,Indonesia

7. Mohammad Ubaidillah: University of Jember,Jember, East Java,Indonesia

Abstract:

Melatonin is a significant source of antioxidants and influences plant growth and development. In this research, we investigated the effect of different concentrations of melatonin on the efficiency of in vitro regeneration and gene expression during morphogenesis in rice (Oryza sativa L.). Data were recorded on the callus induction and regeneration phases to reveal the effects of melatonin treatments resulted in the highest rate of regeneration and gene expression in the varieties used. The results showed that 10 µM of melatonin promoted the regeneration frequency of Cigeulis (71%) and Ketan Hitam (68%) while 15 µM of melatonin promoted the regeneration frequency of TN1 (61%) and Gogo Niti II (76%). Based on PCR analysis, exogenous application of melatonin at 10 and 15 µM showed high expression of OsSERK, OsLEC1 and OsWOX4 genes than those without treatment, while OsBBM gene was not expressed in all treatments. Melatonin treatment during morphogenesis caused a positive response to generating planlets and gene expression.

Page(s): 193-200

DOI: 10.17957/IJAB/15.2019

Published: Journal: International Journal of Agriculture and Biology, Volume: 29, Issue: 3, Year: 2023

Keywords:

melatonin , Rice , in vitro , Gene expression , Morphogenesis

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