Abstract:
Possible use of a vector method of representation of enzymatic reactions is considered in the four and five-dimensional (K`mV`E`ani) and (K`mV`E`aA`ni) coordinate systems for data processing of a simultaneous course of change in four K`m, V`, E`a, ni and five K`m, V`, E`„ A`, ni) parameters of inhibition of the mutant forms of Escherichia coli E15 alkaline phosphatase modified by substitution of neutral amino acid residues in two N-terminal peptides by positively charged lysine residues (Lys, Lys) in the positions (+2+3), (+5+6), (+13+14), (+19+20) and arginine ones (Arg, Arg) in the positions (+5+6), (+13+14), (+19+20). It is established that at transfer of positive charges to the enzyme active centre the maximum inhibitory effect of enzyme activity occurs at substitution in the position (+13+14) from the N-terminus. This effect intensifies with increasing charge of amino acid residues (Lys→Arg ).
Page(s):
633-636
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Biological Sciences, Volume: 5, Issue: 5, Year: 2005