Male Infertility due to Mutation in Human Y Chromosome in Southern Punjab, Pakistan | [Abstract Book on Global Science Technology and Management Conference • 2023]

Author(s):

1. Mashal Rasheed: Department of Biotechnology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

2. Saima Majeed: Department of Biotechnology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

3. Majeeda Rasheed: Department of Life Sciences, KFUE&T, Rahimyar Khan, Pakistan

4. Farkhanda Yasmin: Department of Biochemistry, University of Okara, Okara, Pakistan

Abstract:

To investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility in Pakistani population cross-sectional research was conducted to determine the frequency of y chromosomal microdeletions in infertile males. The study included male patients with infertility issues from a variety of hospitals and infertility clinics throughout three of the most populous districts in Pakistan in southern Punjab region. Participants were adults who had been diagnosed with spermatogenic insufficiency. DNA was extracted from peripheral blood samples and PCR carried out and positive result was scored when the amplification product of expected size was obtained. Our study indicated that 42% of infertile men had Y chromosome AZF microdeletions. Due to the incidence of Y chromosome microdeletions in azoospermic and oligozoospermic males, genetic testing and counselling are indicated before assisted reproduction. After Klinefelter’s illness, Y chromosomal microdeletions caused the greatest male infertility. Infertility caused by azoospermia or oligospermia should be identified using these tests. To avoid unnecessary surgery, patients might have their chromosomes and Y chromosomes microdeletion analyzed. Understanding AZF microdeletions, particularly their prevalence and traits, is crucial to diagnosing and treating oligospermia and azoospermia. AZF, s microdeletion is the most prevalent. Before ICSI and IVF, infertile men should undergo genetic screening to prevent chromosomal diseases like AZF microdeletions from transmitting to their children. Fathers with microdeletions are anticipated to pass on the deletion and reproductive issues to their kids. Therefore, couples, especially those considering assisted reproduction, need genetic counselling and routine Yq microdeletion screening.

Page(s): 73-73

DOI: DOI not available

Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023

Keywords:

Polymerase Chain Reaction , Male infertility , Oligozoospermia , Azoospermia , Y Chromosome deletions Male Infertility , Y chromosome microdeletions

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