Extraction, Phytochemical Screening and Wound Healing Activity of Herbal Formulation of Saussurea lappa | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2019]

Author(s):

1. Aitazaz Ahsan: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

2. G. A. Miana: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

3. Humaira Naureen: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

4. Masood Ur Rehman: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

5. Kamil Anum: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

6. Imran Malik: Riphah Institute of Pharmaceutical Sciences, Riphah International University,Islamabad,Pakistan

Abstract:

The aim of present research work was to develop emulgel and In-situ gels of methanolic extract of Saussurea lappa with the purpose to determine their wound healing and anti-bacterial properties. Phyto-chemical analysis of extract was also performed. Emulgel was prepared by using carbopol 940, Span 80, tween 80, polyethylene glycol (PEG 4000) and methyl paraben while in-situ gel was prepared by using polaxomer (P407) as thermo-sensitive and carbopol (934P) as pH sensitive polymers. All formulations were maintained at pH 6-7 and stored at 4 ?C. Lyophilized extract was added in solution form to enhance the solubility as well as the stability. In-vitro release profile was performed by using Franz diffusion method and data was plotted in different pharmacokinetic models like first order, higuchi and hixon-crowell models. All formulations followed first order release mechanism. Hemolytic activity of extract was performed at concentration of 100µg/ml through heat induced hemolysis of erythrocyte membrane model system while anti-bacterial activity was determined by using agar well diffusion method. Acute toxicity assay of crude extract showed that 1000 mg/kg was safe dose with no toxic symptoms. Excision wounds were induced and wound healing potential of all formulations was determined. Results were compared and expressed as mean ± SEM, and data was analyzed by one way analysis of variance (ANOVA) with p<0.05.

Page(s): 83-96

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 56, Issue: 3, Year: 2019

Keywords:

wound healing , Emulgel , Herbal formulation , Insitu gel , Saussurea lappa

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