A New, Rapid, Cost-Effective, Easy and Validated RP-HPLC Method for Determination of Antiviral (Sofosbuvir) in Bulk Forms. | [Pakistan Journal of Analytical & Environmental Chemistry • 2019]

Author(s):

1. Yasir Mehmood: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

2. Ikram Ullah Khan: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

3. Yasser Shahzad: Department of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan.

4. Syed Haroon Khalid: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

5. Sajid Asghar: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

6. Abid Mehmood Yousaf: Department of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan

7. Talib Hussain: Department of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan

8. Muhammad Irfan: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

9. Ikrima Khalid: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

Abstract:

A simple, specific, accurate and economic reverse phase liquid chromatographic method was developed for the estimation of Sofosbuvir in all polymorphic form. Mobile phase contains acetonitrile and 0.05M ammonium acetate (pH 7.6) in ratio of 50:50 (v/v). A logarithmic calibration curve was plotted from 10 µgmL-1 to 60 µgmL-1 (r = 0.9989) for sofosbuvir, with the limit of detection (LOD) and the limit of quantification (LOQ) of 0.245 µgmL-1 and 0.816 µgmL-1, respectively. The developed method was highly validated and practiced for the assurance of sofosbuvir raw materials with acceptable and non-objectionable accuracy and precision results (recovery 99–102%, RSD <1.2%, n = 3). Zero significant difference (t-test) was obtained between the overall results of the developed RP-HPLC method.

Page(s): 11-16

DOI: DOI not available

Published: Journal: Pakistan Journal of Analytical & Environmental Chemistry, Volume: 20, Issue: 1, Year: 2019

Keywords:

Developed , Accuracy , Chromatography , Sofosbuvir

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views